- Docente: Barbara Mantovani
- Credits: 1
- SSD: BIO/05
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LS) in Biodiversity and Evolution (cod. 0544)
Learning outcomes
Basic knowledges about the main techniques necessary to perform a molecular analysis in the field of taxonomy, systematics and phylogeny in eukariots: isolation of nuclear, mitochondrial and plastidial DNA, electrophoresis, PCR amplification, bacterial cloning, restriction enzyme digestion; nuclear, mitochondrial and plastidial marker analyses, sequencing. Main software packages to be used for data analyses.
Course contents
analysis of moderately (f.i. ribosomal) or highly (f.i. satellite DNA) repeated sequences from animal genomes:PCR amplification of repeated sequences; cloning; positive clones recognition; sequencing. Data elaborations with Mega, DNAsp, MrBayes.
Readings/Bibliography
to be defined with students
Teaching methods
experiments will be performed in the biology laboratory, data evaluation in the informatic one.
Assessment methods
Oral discussion of scientific papers
Teaching tools
biology and informatics laboratories
Office hours
See the website of Barbara Mantovani