- Docente: Luciana Giardino
- Credits: 3
- SSD: VET/01
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LS) in Animal Biotechnologies (cod. 0459)
Learning outcomes
to get basic knowledges about laboratory animals (rat, mouse), housing and general organization, main neurodegenerative models of deseases, behavioural tests
Course contents
1. introduction
biomedical experiments
in vivo, in vitro, ex vivo, bioinformatic models
bioethical principles in animal experiments (3r)
2. housing:
type
sanitization
identification
standardization of housing procedures
environmental variability
rat and mouse housing
3. laboratory animals: rat and mouse
origin
inbred and outbred strains
main strains and factory breeding
light on-off rhythm and food/water conditions
animal handling
animal welfare: behavioral homeostasis
vital space and socialization
stress and stereotypies
4. transgenic mice
terminology
strains
breeding and housing
introducing mutations
genic characterization
brinster’s growth hormone mouse, gfp transgenic mouse (nagy) and galanin-over-expressing mouse
embryonic stem cells, construct preparation, transfection and selection
chimeric founder
5. standardization of in vivo animal models
6. neurodegenerative diseases: animal models
dementia: immunotoxin injection (192igg-saporine), tg2576
parkinson disease: 6ohda pharmacological lesion
ataxia: hcn1-/- mice
experimental allergic encephalomyelitis as animal model of multiple sclerosis
7.behavioural tests to characterize neurodegenerative animal models
morris water maze
rotarod
fear conditioning
rotational behavior
activity cage
pain threshold to thermic and tactile stimulations in free-moving animal
Readings/Bibliography
Sharp P.E., La Regina M.C., The Laboratory Rat, CRC Press, 1998
Guidelines for the care and use of mammals in neurosciences and behavioral research, National Research Council of the National Academies, 2003
Wolfensohn S., Lloyd M., Handbook of laboratpry animal managemenet and welfare, Blackwell Science, 2001
Teaching methods
lessons and inspection to dedicated laboratories, according to the Italian regulation
Assessment methods
oral examination
Teaching tools
power-point presentation; inspection to dedicated laboratories according to the Italian regulation
Office hours
See the website of Luciana Giardino