04223 - Instrumental Analytical Chemistry

Learning outcomes

Fundamentals of the chromatographic methods for the best understanding of the Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC). Fundamentals of the Capillary Electrophoresis (CE) and Mass Spectrometry (MS). The student will be able to choose the best intrumental parameters in order to optimize the separation and the identification of analites derived from biological materials.

Course contents

Fundamentals of chromatographic methods. Gas Chromatography (GC): principles and instrumentation. GC detectors. Thin Layer Chomatography (TLC): principles and applications. High Performance Liquid Chromatography (HPLC): principles and instrumentation. HPLC detectors. Capillary Electrophoresis (CE): principles and instrumentation. Mass Spectrometry (MS): principles and instrumentation (inlet systems, ionic sources, mass analyzers, mass detectors). GC/MS and LC/MS coupled. The mass spectrum: logical and analogical interpretation of the mass spectra.

Readings/Bibliography

D.A. Skoog, J.J. Leary: Chimica analitica strumentale. EdiSES, Napoli (1995).
K.A. Rubinson, J.F. Rubinson: Chimica analitica strumentale. Zanichelli, Bologna (2002).

Teaching methods

Lectures with support of multimedial material, such as slides, movies, and animations. Practices to the computer and in the laboratory for the theoretical and practical resolution of problems concerning the separation and identification of analites derived from biological materials.

Assessment methods

• Continual assessment (3 written tests).
• Final oral test (if necessary).
• Theoretical resolution of problems concerning analite separations and identifications.
  
• Practical resolution of problems concerning analite separations and identifications.
  

Teaching tools

• Multimedial material (slides, movies, animations)
  
• Link to web sites
• Computer practices
• Laboratory practices

Office hours

See the website of Giorgio Bonaga