Course Unit Page
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Teacher Claudia Zanna
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Credits 1
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Teaching Mode Traditional lectures
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Language Italian
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Campus of Bologna
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Degree Programme Single cycle degree programme (LMCU) in Medicine and Surgery (cod. 8415)
SDGs
This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.
Academic Year 2021/2022
Learning outcomes
At the end of the module the student acquires methodological and application elements useful for integrating the knowledge obtained in the courses of Chemistry and Biochemistry.
Course contents
Molecular techniques for qualitative and quantitative DNA analysis, used in the field of clinical diagnostics, forensics, and research.
PCR and Real-time PCR, Gel electrophoresis, Southern blotting, DNA fingerprinting.
Applications of the indicated techniques. Exercises: kinship test, identification of suspects in forensic genetics, clinical diagnoses (Duchenne muscular dystrophy, Huntington's disease; sickle cell anemia, Sars-COV2 positivity…). Design of primers to amplify genes by PCR using specific softwares
Readings/Bibliography
The material is provided to students during each exercise
Teaching methods
The exercise is introduced by a short theoretical presentation on principles, methods and techniques subject to practical experience or by computer simulation
Assessment methods
The evaluation will take place in the context of the overall evaluation of the C.I. and in particular during the end of year interview
Teaching tools
The exercises will be held in rooms equipped with computers
Office hours
See the website of Claudia Zanna
Alumni