84278 - Cellular Biology and Genetics Laboratory (G3)

Course Unit Page

Academic Year 2019/2020

Learning outcomes

Summarize the principles and applications of relevant cellular, molecular and genetic techniques.

Course contents

Lab activities will consist in:

- amplifying DNA: cell-based DNA cloning (using a specific software)

- mitosis and meiosis animations and tests

- gene structure analysis (using the main biological databases)

- learning the use of the main query commands of the online database Pubmed

- studying a single nucleotide polymorphism (SNP) in a genomic sequence (using a specific software) and DNA extraction

- amplifying DNA: polymerase chain reaction (PCR)

- DNA sequencing (Sanger sequencing)

Readings/Bibliography

Protocols and articles will be provided at the beginning of each lab activity.

Teaching methods

- Lab activities

- Virtual experiences, video, teamwork

- Discussion of some topics.

Minimum attendace requirement: 60% of lessons.

Assessment methods

As described for the Integrated Course.

Teaching tools

- Slides with images and schemes.

- Databases and tutorials available online and analysis softwares.

- Selection of scientific articles

Office hours

See the website of Maria Caracausi