67071 - Bioanalytical Chemistry and Laboratory of Analytical Chemistry

Course Unit Page

Academic Year 2018/2019

Learning outcomes

Use and application of quantitative instrumental analysis to chemical and bioanalytical problem: spettrcoscopic techniques (UV-vis, MS), separative techniques (HPLC and field-flow fractionation), chemiluminescence, enzymatic tests, immunoassays. Different analytical assay format, data elaboration and report.

Course contents

Spectroscopy: Uv-vis, atomic absorption, chemiluminescence.

Separative techniques: HPLC (reverse-phase HPLC, size exclusion chromatography, ionic chromatography); field-flow fractionation

Analysis of complex samples: digestion; matrix effect determination.

Methods for the determination of protein concentration.

Enzymatic analysis: Kinetics of enzyme-catalyzed reactions - Measurement of an enzyme catalitic activity - Measurement of the concentration of an enzymatic substrate.

Immonological methods: Competitive and non competitive immunoassays - Homogeneous and heterogeneous immunoassays - Immobilization of antibodies - Synthesis of enzymatic, fluorescent, or chemiluminescent tracers and detection methods - Development and optimization of an immunoassay.

General aspects for gene analysis.

Readings/Bibliography

L. Spandrio "Principi e Tecniche di Chimica Clinica", Piccin
-A.J. Ninfa, D.P. Ballou "Metodologie di Base per la Biochimica e la Biotecnologia", Zanichelli (consultazione)
-J.M. Berg, J.L. Tymoczko, L. Stryer "Biochimica", V Edizione, Zanichelli
Analytical Chemistry by Open Learning: Quantitative Bioassay, D. Hawcroft, T. Hector, F. Rowell, John Wiley and Sons i Dosaggi immunoenzimatici, E. Ishikawa, T. Kawai, K. Miyai, Piccin

Teaching methods

Room lessons assisted by projected material available to students on electronic media from the web.

Training in laboratory

Data elaboration and report preparation

Assessment methods

The final exame grade is the sum of points assigned to the lab reports and the exam evaluation. The exam will be at the end of the all lessons and it is written on topics regardibg laboratory activities and arguments discussed during the class lessons.

Teaching tools

Power Point slides and Word report available on the net http://campus.unibo.it/

Office hours

See the website of Barbara Roda