- Docente: Maurizio Recanatini
- Credits: 9
- SSD: CHIM/08
- Language: Italian
- Moduli: Maurizio Recanatini (Modulo 1) Manuela Bartolini (Modulo 3) Matteo Masetti (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 3) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Pharmaceutical Biotechnology (cod. 8519)
Course contents
Module 1 (4 CFU: 32 h, theory): RATIONAL DRUG DESIGN:
introduction to Medicinal Chemistry and rational drug design.
The process of action of a drug and its phases.
Molecular targets of drugs action: interactions between bio-active
molecules and molecular targets.
Drug science: the classic paradigm and beyond.
Rational design of drugs and introduction to computational
methods.
Module 2 (2 CFU: 30 h,computational lab.): LABORATORY OF
COMPUTATIONAL METHODS: target-based drug design
Introduction to informatic tools used to study drug-protein
interactions.
Analysis of crystal complexes representative of the main classes of
target proteins: enzymes (cyclooxygenases, proteases, kinases); ion
channels (sodium and potassium channels); receptors (GPCRs).
Application of docking techniques to the prediction of drug-target
interactions.
Module 3 (3 CFU: 16 h, theory;12 h, practice bioanalytical
lab.): PHARMACEUTICAL PROTEOMICS
Proteomic analysis strategies. Analytical methodologies in
functional proteomic studies: a) polarized light spectroscopy,
study of the biomolecular recognition mechanism, functional
conformational transitions of monomeric and oligomeric proteins; b)
mass spectrometry in functional proteomics; c) optical biosensor;
characterization of the protein/protein interaction and of higher
order structures; d) affinity chromatography; immobilization of
soluble proteins, enzymes, and membrane receptors; e) hyphenated
techniques, HPLC/MS/MS, HPALC/ HPLC/MS/MS, HPLC/CD, HPLC/MS
/optical biosensor; specific applications to ligand fishing,
post-translational modification, enzyme inhibitor screening and
modulation of protein/protein interaction.
Assessment methods
The final examination will evaluate the knowledge of the contemporary approaches to the design of bio-active small molecules, of some molecular modeling methods for the drug design, and of analytical methods used to study the functional relationships between expressed proteins and their effect on cellular processes. The modules will be evaluated separately (1 and 3 orally, 2 experimentally in the computational lab) and the final score will be calculated as the average of the scores of the single modules.
Office hours
See the website of Maurizio Recanatini
See the website of Manuela Bartolini
See the website of Matteo Masetti
SDGs
This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.