00062 - Applied Biochemistry

Learning outcomes

At the end of the course the course's attendant: - will learn the basic knowledge of the main biochemistry technologies and analytical methodologies; - will understand the rationale of the application of these technologies to medicine and clinic; - will be able to apply the biochemistry methodologies to the principal clinical analyses and to understand and correctly interpreter the results in a diagnostic/prognostic perspective;

Course contents

Applied Biochemistry I (24 hr) Prof. Paolo Neyroz

 1. UV-VIS spectroscopy

a) The Lambert-Beer law

2.Fluorescence spectroscopy 

3. Chemiluminescence 

4. Principles of  cytofluorometry

5. Liquid chromatography 

a) gel-filtration

b) ionic exchange

c) affinity chromatography

6. Gel electrophoresis 

7. Immunochemistry 

8. Molecular biology techniques

Applied Biochemistry II (20 hr) Prof. Concettina Cappadone

Spectrophotometric methods: ultraviolet and visible light spectroscopy (principles, instrumentation, applications).

Spectrophotometric analysis of protein concentration.

Enzyme kinetics: analysis  of alkaline phosphatase activity; effect of substrate concentration, graphs construction and measure of Km and Vmax

Readings/Bibliography

 Biochimica Applicata, autori Stoppini-Bellotti Editore EdiSES, Napoli 
 

Metodolgia biochimica - Le bioscienze e le biotecnologie in laboratorio

A cura di Keith Wilson e John Walker

Edizioni Raffaello Cortina Editore, Milano costo: 98.13€

Biochimica Clinica

Luigi Spandrio

Edizioni Sorbona, Milano costo: 58€ 

Assessment methods

To have access to the examination test it is needed to sign into the electronic panel within the scheduled dates. Those which could not sign must send an email to the lecturer (before the closing date) to communicate their problem. Depending on the lecturer decision the student will be admitted to the test.
Students evaluation will be provide by a final examination to verify the appropriate preparation on the different course's topics. 
The final test will be given as oral examination of two parts: A first part with 3 questions regarding the theoric topics of the course and a second part of 1 question regarding the laboratory practice activities performed during the course (Module of Applied Biochemistry II).
The result of the evaluation is based on the answers to the tests and the exhibited  acquaintance of the course's fundamentals to fulfill the didactic objectives. The final definition of the student preparation and capabilities is given in a score as a fraction of thirty (from 18/30 to 30/30 cum laude).

Teaching tools

Powerpoint slides

Office hours

See the website of Paolo Neyroz

See the website of Concettina Cappadone