- Docente: Manuela Donati
- Credits: 1
- SSD: MED/07
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Long cycle 2nd degree programme in Medicine (cod. 0080)
Learning outcomes
The aim of the Course is to give basic concepts and tools for the use of immunofluorescence test in the laboratory diagnosis of bacterial infections with particular regard to those infections in which immunofluorescence represents the most employed diagnostic test.
At the end of the Course the student should be able to know the situations in which immunofluorescence test has to be used and should understand the diagnostic importance of the results obtained after examination at a UV microscope.
Course contents
Serological reactions.
Techniques used for producing monoclonal antibodies and polyclonal immune sera.
Use of direct and indirect immunofluorescence tests to detect specific antigens and antibodies in bacterial infections, with particular regard to the infections caused by Chlamydiae, Borreliae, Rickettsiae, Legionellae.
Use of immunofluorescence test to investigate the in vitro activity of antibiotics against chlamydiae.
Use of microimmunofluorescence assay in the serological diagnosis of chlamydial infections.
Readings/Bibliography
La Placa - "Principi di Microbiologia medica" - last edition.
Teaching methods
Theoretical lectures supported by laboratory activities.
Assessment methods
Oral examination.
Teaching tools
Documentation provided by the teacher.
Links to further information
http://www.webmed.unibo.it/programmi_didattici/elettivi/elettivodonati2006.html
Office hours
See the website of Manuela Donati