Method for the analysis of secreted and/or endocited from cells in culture particles

The invention relates to two types of cuvettes, suitable to be used in the DLS instrument and z-potential, that allow the real-time measurement of cell activity products (eg. Exosomes).

Title of the patent Method for the analysis of secreted and/or endocited from cells in culture particles
Thematic area Chemistry and biotechnology
Ownership Alma Mater Studiorum – University of Bologna
Inventors Alice Galbiati
Protection International (PCT)
Licensing status Available for licensing agreement
Keywords Cell adhesion layer, seed channel, cover, on/off switch, exosomes
Filed on Oct 03, 2016

The cuvettes currently on the market are not designed to allow cell growth, hence the origin of this invention, which allows researchers to analyze cellular phenomena during time.

The invention consists of two types of cuvettes that allow researchers to study particular cellular phenomena in real time and during different timeframe, while maintaining the integrity of the cell sample. Cuvettes allow cell adhesion on the bottom and the possibility of recovering the cell sample after the analysis, as well as maintain sterility from the incubator to DLS instrument (in the case of the cuvette by size). Furthermore, the cuvette for z-potential allow the sowing of cells in the cuvette and the opening/closing of the channel that connects the cells with the culture medium.

Advantages: possibility of cultivating cells on the bottom of the cuvette, allowing real-time analysis of the dimensions of the surface potential, of  the structures produced by the cells (eg. Exosomes) and monitoring the up-take of real-time nano and micro particles produced by the cells and several other applications in specific studies.