Catalyst additive for hybridization reactions

The catalyst additive reduce the In Situ Hybridization duration up to 157-165 minutes compared to standard (2/3 days). It can be added to commercial probes for hybridization on histological and cytological samples.

Title of the patent Catalyst additive for hybridization
Thematic area Chemistry and biotechnology
Ownership Alma Mater Studiorum - Università di Bologna
Inventors Enrico Di Oto, Valentina Monti, Sofia Asioli
Protection Europe, USA, China, India
Licensing status Available for licensing agreement
Keywords Fluorescent In Situ Hybridization (FISH), cancer, breast, lung, brain, thyroid, HER-2, target therapy, diagnosis
Filed on Feb 04, 2015

In the field of molecular techniques, In Situ Hybridizations are widely used in order to define the profile of  diagnostics, prognostics and predictive genetic markers. Between all In Situ Hybridization methods, FISH technique is considered to have the highest sensibility and sensitivity but as disadvantage it requires 2/3 days for obtaining an outcome. This causes a discomfort for the patient waiting for the outcome of the analysis, and it delays the start of adequate therapeutic approach.

The present invention is applied in molecular analysis especially in the field of DNA/RNA aberrations investigated by In Situ Hybridization techniques. The patented catalyst additive is a mixture designed to minimize the analysis overall time from 2/3 days to few hours.

The patented catalyst additive can be used  for  a variety of biological samples, particularly breast, lung, brain and thyroid human tissues.

Main advantages:

  •     Overall time strongly reduced up to 157 minutes
  •     Low cost reagents
  •     Applicable to most laboratory protocols
  •     Can be used in combination with commercial probes
  •     Formammide free composition