- Docente: Marco Lucarini
- Credits: 9
- SSD: CHIM/06
- Language: Italian
- Moduli: Stefano Masiero (Modulo 1) Marco Lucarini (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Chemistry and Pharmaceutical Technologies (cod. 8412)
Learning outcomes
Aim of the course is to provide the student with the knowledge
necessary to identify and characterize an organic compound by
using spectroscopic techniques.
In order to follow profitably the course the student must know the
basic principles of organic chemistry as well as of physics.
Course contents
Quantization of the energy levels of atoms and
molecules. Absorption of electromagnetic radiations by
molecules.
UV and visible spectroscopy. Beer-Lambert law. Electronic
transitions and Molecular Orbitals. Absorbing groups: polyenes,
aromatic rings, carbonyl compounds. Substituent and solvent effects
on electronic transitions. Structural information from UV and
visible spectra. Applications.
Linear and circular dichroism: outlines.
Fluorescence spectroscopy. Excited electronic states and
Jablonski diagram. Fluorescence and phosphorescence. Applications.
Infrared spectroscopy. Harmonic and anharmonic oscillators.
Selection rules for vibrational transitions. Group frequencies and
analysis by IR.
Nuclear Magnetic Resonance. Zeeman interaction between
nuclear spin and magnetic field. Spin-spin coupling. NMR spectra of
AX and AB systems. First order spectra. Chemical shift and chemical
structure. Factors determining the magnitude of the spin-spin
coupling constants. Interpretation of simple proton NMR spectra.
Carbon-13 NMR spectra. The sensitivity in NMR spectroscopy.
Pulsed NMR and Fourier transform. Proton decoupling. Chemical shift
of carbons. Applications.
Mass spectrometry. Principles and outlines of
instrumentation. Ionisation by electron impact and chemical
ionisation. Mass analysers. Molecular ion and isotopomers. Modes of
fragmentation. Fragmentation of common functional groups.
Readings/Bibliography
G.F. Pedulli, Metodi Fisici in Chimica Organica, Piccin
Editore, Padova, 1996.
Teaching methods
Lectures and training lessons
Assessment methods
Solution of an exercise consisting in the identification of an
unknown substance on the basis of the IR, UV, proton and 13C NMR,
and Mass spectra.
Written exam on the theoretical and practical aspects of the
various spectroscopic techiques.
Teaching tools
PowerPoint slides
Office hours
See the website of Marco Lucarini
See the website of Stefano Masiero