- Docente: Angela Rampa
- Credits: 4
- SSD: CHIM/08
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Applied Pharmaceutical Sciences (cod. 0914)
Course contents
Introduction to thin layer chromatography: methods for the analysis and isolation of different classes of natural products. Preparative thin layer chromatography. Laboratory protocols for extraction of specific classes of natural products. Purification by solvent extraction using partition coefficient. Ionic solvents. Methods of separation of natural compounds for countercurrent chromatography. Scale-up of purification of natural compounds. Purification of water soluble natural products: resins, silica gel C18, stationary phase. Applications and limitations of non-ionic resins. Purification by crystallization of natural products with low molecular weight. Fractional crystallization. Laboratory experiments: -purification of terpenes: camphor and borneol from aerial parts of Cinnamomum camphora and Rosmarinum officinalis; - extraction and purification of salicylic acid from bark of Salix alba;- purification of optically active organic basis for fractional crystallization from a racemic mixture; - purification by preparative chromatography of alkaloids contained in the bulb of Narcissus pseuronarcissus cv. Carlton; - purification of ginsenoside from Panax ginseng using the methods of FU; - purification by steam distillation of eugenol contained in Pimenta officinalis.
Readings/Bibliography
1) VOGEL. CHIMICA ORGANICA PRATICA. CASA EDITRICE AMBROSIANA, SECONDA EDIZIONE, MILANO 1988 2) R. M. ROBERTS, J.C. GILBERT, S. F. MARTIN. CHIMICA ORGANICA SPERIMENTALE. ZANICHELLI, BOLOGNA 2003 3) A.J. HANDELY. EXTRACTION METHODS IN ORGANIC ANALYSIS. SHEFFIELD ACADEMIC PRESS, CANADA 1999 4) D.L. PAVIA, G. M. LAMPMAN, G.S. KRIZ. IL LABORATORIO DI CHIMICA ORGANICA, EDIZIONI SORBONA , MILANO 1994 5) P.M. DEWICK. CHIMICA, BIOSINTESI E BIOATTIVITA' DELLE SOSTANZE NATURALI. PICCIN, PADOVA 2000 6) MARCO D'ISCHIA LA CHIMICA ORGANICA IN LABORATORIO PICCIN PADOVA 2002 LUCIDI, CD, APPUNTI DI LEZIONI, COPIE DI ARTICOLI INERENTI GLI ARGOMENTI DI LEZIONE 7) PAUL H. LIST, PETER C. SCMIDT I FARMACI DI ORIGINE VEGETALE TECNOLOGIE DI ESTRAZIONE DELLE PIANTE MEDICINALI, HOEPLI 1993 8) NATURAL PRODUCT ISOLATION: An Overview Book Series Methods in Biotechnology ISSN 1940-6061 (Print) 1940-607X (Online) Volume Volume 20 Edition Second Edition.
Teaching tools
VIDEOPROJECTORS, PC, TRASPARENCIES, LABORATORY OF CHEMISTRY
Office hours
See the website of Angela Rampa