- Docente: Carlo Bertucci
- Credits: 3
- SSD: CHIM/08
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Long cycle 2nd degree programme in Chemistry and Pharmaceutical Technologies (cod. 0038)
Learning outcomes
The course is aimed to provide an overview focused on the role played by analytical methodologies in drug discovery and development. The course should allow learning about the molecular recognition mechanisms, and, in particular, about the drug/target protein interaction process, by making use of analytical methodologies.
Course contents
1. High Performance Affinity Liquid Chromatography (HPALC). Immobilization of receptors, enzymes and plasma proteins in the characterization of new compounds for their activity and their distribution parameters. Determination of binding parameters. Displacement chromatography. Reversible and covalent modification of the protein.
2. Polarized light spectroscopy. Secondary structure of proteins, and characterization of drug/protein and protein/protein complexes. Determination of binding parameters. Competitive binding. Stereochemical characterization of the bound drug.
3. Optical biosensors. Screening of new compounds for their activity and ADME parameters. Kinetics of the molecular recognition process.
4. Hyphenated techniques for the functional and structural characterization of analytes.
Lectures will be followed by laboratory experiments on the use of immobilized protein for studying the drug/protein process.
Readings/Bibliography
1). I.W.Wainer, Drug Stereochemistry, Analytical Methods and Pharmacology, M.Dekker, 1993. 2). T.Cass, F.S.Ligler, Immobilized Biomolecules in Analysis, Oxford University Press, 1998. 3). Lecture slides and specific articles and reviews
Teaching methods
Lectures and laboratory
Assessment methods
Oral examination
Teaching tools
Personal computer and video-projector.
Office hours
See the website of Carlo Bertucci