Foto del docente

Stefania Trazzi

Senior assistant professor (fixed-term)

Department of Biomedical and Neuromotor Sciences

Academic discipline: BIO/09 Physiology

Curriculum vitae

PERSONAL DETAILS

First Name: Stefania

Last Name: Trazzi

Born: Bologna on October 5th 1970

Professional address: Department of Biomedical and Neuromotor Sciences, University of Bologna, Unit of Physiology, Piazza di Porta San Donato 2, 40126 Bologna Italy.

Laboratory Phone: 0039-051-2091760.

e-mail: stefania.trazzi3@unibo.it

 

EDUCATION

1998: Degree in Biological Sciences (110/110 Cum Laude), University of Bologna, Italy.

2002: Specialization in Applied Genetics (70/70 Cum Laude), University of Bologna, Italy.

2006: Ph.D. in Cell Biology and Physiology at the University of Bologna.

 

RESEARCH EXPERIENCES AND TRAINING

September 1999-August 2002: 3-years postgraduate research Scholarship: “Construction of human artificial chromosomes through the assembly of essential elements”; Department of Biology, University of Bologna; Tutor: Prof. Giuliano Della Valle.

June 1999: Workshop Fluorescence in situ hybridization. Prof. Mariano Rocchi. University of Bari, Italy.

June 2000: Workshop Genome structural and functional analisys. Italian Association of Genetic and Genetic School of Cortona Italy.

January to April 2006: professional services contract: Title: “Identification of new molecular markers of neuronal cell death in Alzheimer's disease”; Department of Experimental and Diagnostic Medicine, University of Ferrara; Tutor: Prof. Rosario Rizzuto.

May 2006-January 2007: 9-months post-doctoral fellowship: Title: “Identification of new molecular markers of neuronal cell death in Alzheimer's disease”; Department of Experimental and Diagnostic Medicine, University of Ferrara; Tutor: Prof. Rosario Rizzuto.

January 2007-January 2008: 1-year post-doctoral fellowship: Title: “Defining the pathogenesis of Facio-Scapulo-Humeral Dystrophy (FSHD)”; Department of Biomedical Sciences, University of Modena and Reggio Emilia [http://en.wikipedia.org/wiki/University_of_Modena_and_Reggio_Emilia] ; Tutor: Prof. Rossella Tupler.

February 2008-May 2011: 4-years post-doctoral fellowship: Title: “Effect of nervous signals on hippocampal neurogenesis”; Department of Physiology, University of Bologna; Tutor: Prof. Renata Bartesaghi.

June 2011-September 2011: 4-months contract of collaboration (Co.Co.Co):

Title: “Effect of prenatal and postnatal treatment with fluoxetine on neurogenesis and dendritic maturation of Ts65Dn mice, a mouse model of Down syndrome”; Department of Physiology, University of Bologna; Tutor: Prof. Renata Bartesaghi.

October 2011-November 2012: 1-year post-doctoral fellowship:

Title: “Role of hypothalamic neurogenesis in the development of obesity and its cardiovascular comorbidities”; Department of Biomedical and Neuromotor Sciences, University of Bologna; Tutor: Prof. Renata Bartesaghi.

December 2012-December 2015: 2-years post-doctoral fellowship:

Title: “Molecular mechanisms underlying brain alterations in the CDKL5 variant of Rett's syndrome”; Department of Biomedical and Neuromotor Sciences, University of Bologna; Tutor: Prof. Renata Bartesaghi.

January 2015-December 2016: 2-years contract of collaboration (Co.Co.Co):

Title: "Encephalopathy CDKL5: therapeutic strategies to improve brain development in a CDLK5 knockout mouse model"; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna; Tutor: Prof. Elisabetta Ciani.

January 2017-March 2017: 1-years post-doctoral fellowship:

Title: “Identification of CDKL5’s molecular targets”; Department of Biomedical and Neuromotor Sciences, University of Bologna; Tutor: Prof. Elisabetta Ciani.

April 2017: Assistant Professor, Department of Biomedical and Neuromotor Sciences, University of Bologna

 

AWARDS

2002: Productivity prize, Department of Biology University of Bologna, for the structural and functional analysis of centromeric chromatin: interactions’ study between CENP-C and centromeric DNA and in vivo characterization of sequences recognized by CENP-C and CENP-B in human conventional centromeres and neo-centromeres.

2005: Productivity prize, Department of Biology University of Bologna, for the Identification of N-Myc target genes in neuroblastoma cells.

2006: Productivity prize, Department of Biology University of Bologna, for the study of DNA/Proteins interactions in centromere/kinetochore complex: functional analysis of CENP-C protein.

 

TEACHING EXPERIENCE

2001: Contract as laboratory instructor, “Genetic II”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2002: Contract as laboratory instructor, “Genetic II”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2003: Contract as laboratory instructor, “Genomics”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2003: Contract as laboratory instructor, “Genetic II”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2004: Contract as laboratory instructor, “Genetic II”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2005: Contract as laboratory instructor, “Genetic II”. 1st level Degree in Biological Science. Dept. of Biology, University of Bologna.

2016: Contract as laboratory instructor, “Physiology”. Degree in Dentistry. Dept. of Biomedical and Neuromotor Sciences, University of Bologna.

 

SUPERVISION OF UNDERGRADUATE AND GRADUATE STUDENTS

2004: Undergraduate student (Luigi Pasini) Department of Biology, University of Bologna.

2007: Undergraduate student (Monica Zoli) Department of Biology, University of Bologna.

2008: PhD student (Simona Rizzi) Department of Physiology, University of Bologna.

2008: Undergraduate student (Sebastiano Cristiani) Department of Physiology, University of Bologna.

2008: Undergraduate student (Barbara Corelli) Department of Physiology, University of Bologna.

2008: Undergraduate student (Daniela Felice) Department of Physiology, University of Bologna.

2009: Undergraduate student (Marco Togni) Department of Physiology, University of Bologna.

2009: Undergraduate student (Claudia Fuchs) Department of Physiology, University of Bologna.

2010: PhD student (Patrizia Bianchi) Department of Physiology, University of Bologna.

2010: Undergraduate student (Eugenia Giglio) Department of Physiology, University of Bologna.

2010: Undergraduate student (Annalisa Alfieri) Department of Physiology, University of Bologna.

2010: Undergraduate student (Michele Fedecostante) Department of Physiology, University of Bologna.

2011: Undergraduate student (Elena Ragazzi) Department of Physiology, University of Bologna.

2011: Undergraduate student (Carlo Pieroni) Department of Physiology, University of Bologna.

2011: Undergraduate student (Marianna De Franceschi) Department of Physiology, University of Bologna.

2012: PhD student (Valentina Maria Mitrugno) Department of Physiology, University of Bologna.

2013: Undergraduate student (Riccardo Ramini) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2013: Undergraduate student (Andrea Giacomini) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: Undergraduate student (Tiziana Uras) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: Undergraduate student (Marco Emili) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: PhD student (Fiorenza Stagni) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: Undergraduate student (Natalia Cappoli) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: PhD student (Claudia Fuchs) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2014: Undergraduate student (Rocchina Assunta Viggiano) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2016: PhD student (Giorgio Medici) Department of Biomedical and Neuromotor Sciences, University of Bologna.

2020: Undergraduate student (Nicola Mottolese) Department of Biomedical and Neuromotor Sciences, University of Bologna.

 

NATIONAL AND INTERNATIONAL MEETINGS

Speaker

  1. Trazzi S., Strippoli P., Tonelli R., Morrica M., Messina C., Guizzunti G., Tafuri A. and Bagnara G.P. Molecolar analysis of G-CSF receptor in acute myeloid leukemia. 5° Meeting Italian Association of Experimental Haematology, Rome, Italy 1998.
  2. Trazzi S., Politi V., Perini G., Bernerdoni R., Pliss A., Raska I., Earnshaw WC. and Della Valle G. Protein/DNA interactions in centromere/kinetochore complex. 4° Meeting Italian Association of Life Science FISV, Riva del Garda (TN), Italy 2002.
  3. Trazzi S., Perini G. and Della Valle G. Dissecting CENP-C function in mammalian centromeres. 7° Meeting Italian Association of Life Science FISV, Riva del Garda (TN) Italy 2005.
  4. Trazzi S., Rizzi S., Cristiani S., Bartesaghi R. and Ciani E. Neuronal stem cell cultures from the Ts65Dn mouse, an animal model for Down syndrome, represent a suitable system for dissecting the molecular mechanisms underlying neurogenesis impairment. 59° Meeting Italian Association of Physiology, Cagliari, Italy 2008.
  5. Trazzi S., Valli E., Fuchs C., Bartesaghi R., Perini G. and Ciani E. Coinvolgimento di CDKL5 nei processi di proliferazione e differenziamento neuronale. Italian CDKL5 Foundation Meeting, Milan, Italy 2010.
  6. Trazzi S. CDKL5: a key regulator of neuronal proliferation and differentiation. I° International Meeting on CDKL5 rare disease. Bologna, Italy 2012.
  7. Trazzi S. Pharmacological therapeutic approaches for cdkl5 disorder: HDAC4: a key factor underlying brain developmental alterations in CDKL5 disorder. 3rd International CDKL5 Research Symposium. Itasca, Illinois, USA 2016.
  8. Trazzi S., Fuchs C., Viggiano R., Jedynak P., De Franceschi M., Medici G., Fustini N., Kurz T., Bartesaghi R. and Ciani E. HDAC4: a possible therapeutic target to improve brain developmental alterations in CDKL5 disorder. Focus on CDKL5 Symposium. Turin, Italy 2016.
  9. Trazzi S: Pharmacological approaches to rescue brain development in CDKL5 disorder. 4° International CDKL5 Congress, Roma, Italy 2017.
  10. Trazzi S., Galvani G., Fuchs C., Gennaccaro L., Ciani E. Loss of CDKL5 enhances microglia activation: are neuro-inflammatory mechanisms implicated in the pathophysiology of CDKL5 deficiency disorder? 1st International Conference on Neuroprotection by Drugs, Nutraceuticals and Physical Activity. Rimini, Italy 2019.

RESEARCH PROJECTS

2008: Two years national research grant PRIN (Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale, Italy) entitled: "Early pharmacotherapy for correcting impairment of brain development in the Ts65Dn mouse, a model for Down syndrome". Co-investigator.

2009: Two years national research grant (Carisbo Foundation, Italy) entitled: "Ruolo della Neurogenesi Ipotalamica Nello Sviluppo Dell’obesità E Delle Sue Comorbidità Ipniche E Cardiovascolari". Co-investigator.

2009: Two years international research grant (Jérôme Lejeune Foundation, France), entitled: “Early pharmacotherapy for correcting impairment of brain development in the Ts65Dn mouse model for Down syndrome”. Co-investigator.

2009: One year national research grant (Italian CDKL5 Foundation, Italy), entitled: “CDKL5 variant of Rett's syndrome: molecular and therapeutic approaches”. Co-investigator.

2010: Two years national research grant (Del Monte Foundation, Italy), entitled: “Molecular mechanisms underlying brain alterations in the CDKL5 variant of Rett's syndrome”. Co-investigator.

2011: Two years international research grant (Jérôme Lejeune Foundation, France), entitled: “Early pharmacotherapy to rescue brain development and cognitive performance in Down syndrome”. Co-investigator

2011: Two years national research grant (Del Monte Foundation, Italy), entitled: “CDKL5 variant of Rett's syndrome: molecular and therapeutic approaches”. Co-investigator.

2011: Three years national research grant (Telethon Foundation, Italy), entitled: “Molecular mechanisms underlying brain alterations in the CDKL5 variant of Rett’s syndrome”. Co-investigator

2012: One year international research grant (UK CDKL5 Foundation, UK), entitled: “CDKL5 variant of Rett's syndrome: protein substitution therapy”. Co-investigator.

2013: Three years national research grant (Telethon Foundation, Italy) entitled: “Preventive therapy of mental retardation in Down syndrome by a novel gamma-secretase inhibitor: focus on APP-dependent mechanisms in neurodevelopment”. Co-investigator.

2013: Two years national research grant (Del Monte Foundation, Italy), entitled: “CDKL5 encephalopathy: a new therapeutic approach to improve brain development in a knockout mouse model for CDKL5”. Co-investigator.

2014: Two years international research grant (Jérôme Lejeune Foundation, France.), entitled: “CDKL5 encephalopathy: therapeutic strategies to improve brain development in a newly generated Cdkl5 knockout mouse model”. Co-investigator.

2015: Three years national research grant (Fondazione e Assicurazione Generali, Italy) entitled: "New avenues for the rescue of intellectual disability in Down syndrome". Co-investigator.

PUBLICATIONS

  1. Politi V., Perini G., Trazzi S., Pliss A., Raska I., Earnshaw WC. and Della Valle G. 2002. CENP-C binds the alpha satellite in vivo at specific centromere domains. Journal of Cell Science, 115:2317-27.
  2. Trazzi S., Bernardoni R., Diolaiti D., Politi V, Earnshaw WC, Perini G. and Della Valle G. 2002. in vivo functional dissection of the human inner kinetochore protein CENP-C. J Struct Biol. 140:39-48.
  3. Diolaiti D., Bernardoni R., Trazzi S., Bono F., Herbert JM., Perini G., and Della Valle G. 2007. Functional cooperation between TrkA and p75NTR accelerates NGF-mediated neuronal differentiation by increased transcription of GAP43 and p21(CIP/WAF) genes via ERK1/2 and AP-1 activities. Experimental Cell Research. 313(14):2980-92.
  4. Trazzi S., Perini G., Bernardoni R., Zoli M., Reese J.C., Musacchio A. and Della Valle G. 2009. The C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation. PLoS One. 4(6):e5832.
  5. Fila T., Trazzi S., Crochemore C., Bartesaghi R. and Ciani E. 2009. Lot1 negatively regulates neuronal precursor proliferation through the PACAP receptor. J Biol Chem. 284(22):15325-38.
  6. Gopalakrishnan S., Sullivan B.A., Trazzi S., Della Valle G., Robertson K.D. 2009. DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric regions. [http://www.ncbi.nlm.nih.gov/pubmed/19482874] Hum Mol Genet. 18(17):3178-93.
  7. Trazzi S., Steger M., Mitrugno V.M., Bartesaghi R. and Ciani E. 2010. CB1 cannabinoid receptors increase neuronal precursor proliferation through AKT/glycogen synthase kinase-3beta/beta-catenin signaling. J Biol Chem. 285(13):10098-109.
  8. Bianchi P., Ciani E., Guidi S., Trazzi S., Felice D., Grossi G., Fernandez M., Giuliani A., Calzà L. and Bartesaghi R. 2010. Early pharmacotherapy restores neurogenesis and cognitive performance in the Ts65Dn mouse model for Down syndrome. J Neurosci. 30(26):8769-79.
  9. Trazzi S., Mitrugno V.M., Valli E., Fuchs C., Rizzi S., Guidi S., Perini G., Bartesaghi R. and Ciani E. 2011. APP-dependent up-regulation of Ptch1 underlies proliferation impairment of neural precursors in Down syndrome. Hum Mol Genet. 20(8):1560-73.
  10. Fuchs C., Ciani E., Guidi S., Trazzi S., Bartesaghi R. 2012. Early-occurring proliferation defects in peripheral tissues of the Ts65Dn mouse model of Down syndrome are associated with patched1 over expression. Lab Invest. 92(11):1648-60.
  11. Valli E*., Trazzi S*., Fuchs C., Erriquez D., Bartesaghi R., Perini G. and Ciani E. 2012. CDKL5, a novel MYCN-repressed gene, blocks cell cycle and promotes differentiation of neuronal cells. Biochim Biophys Acta. 1819(11-12):1173-1185 *co-first authors.
  12. Guidi S., Stagni F., Bianchi P., Ciani E., Ragazzi E., Trazzi S., Grossi G., Mangano C., Calzà L. and Bartesaghi R. 2013. Early Pharmacotherapy with Fluoxetine Rescues Dendritic Pathology in the Ts65Dn Mouse Model of Down Syndrome. Brain Pathol. 23(2):129-43.
  13. Bianchi P., Bettini S., Guidi S., Ciani E., Trazzi S., Stagni F., Ragazzi E., Franceschini V. and Bartesaghi R. 2014 Age-related impairment of olfactory bulb neurogenesis in the Ts65Dn mouse model of Down syndrome. Exp Neurol. 251:1-11.
  14. Trazzi S., Fuchs C., De Franceschi M., Mitrugno V.M., Bartesaghi R. and Ciani E. 2014. APP-dependent alteration of GSK3β activity impairs neurogenesis in the Ts65Dn mouse model of Down syndrome. Neurobiol Dis. 67:24-36.
  15. Fuchs C., Trazzi S., Torricella R., Viggiano R., De Franceschi M., Amendola E., Gross C., Calzà L., Bartesaghi R. and Ciani E. 2014. Loss of CDKL5 impairs survival and dendritic growth of newborn neurons by altering AKT/GSK-3β signaling. Neurobiol Dis. 70:53-68.
  16. Fuchs C., Rimondini R., Viggiano R., Trazzi S., De Franceschi M., Bartesaghi R. and Ciani E. 2015. Inhibition of GSK3β rescues hippocampal development and learning in a mouse model of CDKL5 disorder. Neurobiol Dis. 82:298-310.
  17. Giacomini A., Stagni F., Trazzi S., Guidi S., Emili M., Brigham E., Ciani E. and Bartesaghi R. 2015. Inhibition of APP gamma-secretase restores Sonic Hedgehog signaling and neurogenesis in the Ts65Dn mouse model of Down syndrome. Neurobiol Dis. 82:385-396.
  18. Stagni F., Giacomini A., Emili M., Trazzi S., Guidi S., Sassi M., Ciani E., Rimondini R. and Bartesaghi R. 2016. Short- and long-term effects of neonatal pharmacotherapy with epigallocatechin-3-gallate on hippocampal development in the Ts65Dn mouse model of Down syndrome. Neuroscience., 333:277-301.
  19. Trazzi S., Fuchs C., Viggiano R., De Franceschi M., Valli E., Jedynak P., Hansen F.K., Perini G., Rimondini R., Kurz T., Bartesaghi R. and Ciani E. 2016. HDAC4: a key factor underlying brain developmental alterations in CDKL5 disorder. Hum Mol Genet. 25(18):3887-3907
  20. Guidi S., Bianchi P., Stagni F., Giacomini A., Emili M., Trazzi S., Ciani E. and Bartesaghi R. 2016. Lithium Restores Age Related Olfactory Impairment in the Ts65Dn Mouse Model of Down Syndrome. CNS Neurol Disord Drug Targets. 13:1-8.
  21. Trazzi S., De Franceschi M., Fuchs C., Bastianini S., Viggiano R., Lupori L., Mazziotti R., Medici G., Lo Martire V., Ren E., Rimondini R., Zoccoli G., Bartesaghi R., Pizzorusso T. and Ciani E. 2018. CDKL5 protein substitution therapy rescues neurological phenotypes of a mouse model of CDKL5 disorder. Hum Mol Genet. 27(9):1572-1592.
  22. Fuchs C., Fustini N., Trazzi S., Gennaccaro L., Rimondini R.and Ciani E. 2018. Treatment with the GSK3-beta inhibitor Tideglusib improves hippocampal development and memory performance in juvenile, but not adult, Cdkl5 knockout mice. Eur J Neurosci. 47(9):1054-1066.
  23. Fuchs C., Gennaccaro L., Trazzi S., Bastianini S., Bettini S., Lo Martire V., Ren E., Medici G., Zoccoli G., Rimondini R. and Ciani E. 2018. Heterozygous CDKL5 Knockout Female Mice Are a Valuable Animal Model for CDKL5 Disorder. Neural Plasticity https://doi.org/10.1155/2018/9726950
  24. Ren E*, Roncacé V*, Trazzi S*, Fuchs C, Medici G, Gennaccaro L, Loi M, Galvani G, Ye K, Rimondini R, Aicardi G, Ciani E. 2019. Functional and Structural Impairments in the Perirhinal Cortex of a Mouse Model of CDKL5 Deficiency Disorder Are Rescued by a TrkB Agonist. Front Cell Neurosci. 13:169 (IF2018: 3.014).
  25. Fuchs C, Medici G, Trazzi S, Gennaccaro L, Galvani G, Berteotti C, Ren E, Loi M, Ciani E. 2019. CDKL5 deficiency predisposes neurons to cell death through the deregulation of SMAD3 signaling. Brain Pathol. doi: 10.1111/bpa.12716. (IF2018: 6.187).
  26. Fuchs C, Gennaccaro L, Ren E, Galvani G,Trazzi S, Medici G, Loi M, Conway E, Devinsky O, Rimondini R, Ciani E. 2019. Pharmacotherapy with sertraline rescues brain development and behavior in a mouse model of CDKL5 deficiency disorder. Neuropharmacology doi: 10.1016/j.neuropharm.2019.107746. (IF2018: 4.367).
  27. Loi M*, Trazzi S*#, Fuchs C, Galvani G, Medici G, Gennaccaro L, Tassinari M, Ciani E#. 2020. Increased DNA Damage and Apoptosis in CDKL5-Deficient Neurons. Mol Neurobiol. doi: 10.1007/s12035-020-01890-w. (IF2019: 4.586).

 

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