Foto del docente

Gerardo Manfreda

Full Professor

Department of Agricultural and Food Sciences

Academic discipline: VET/04 Inspection of Foods of Animal Origin

Research

Keywords: Salmonella enteritidis, Salmonella typhimurium, Listeria monocytogenes, Campylobacter jejuni, E.coli detection and quantification by molecular techniques Po and FSO criteria for risk assessment Sampling plan to improve the biological risk assessment metagenomic analysis to identify the microbial profile of poultry intestine antibiotic resistance molecular typing of foodborne pathogens

1) Study of bacterial growth dynamic and  pathogenicity factors of Campylobacter jejuni and Listeria monocytogenes isolated from poultry, pig and dairy products
2) Evaluation of different molecular techniques in order to genetically characterize Helicolbacter pullorum and Clostridium perfringens strains of avian origin collected in the different European countries
3) Identification and sequencing of gyrase gene of Helicobacter pullorum
4) Optimisation and standardisation of ELISA tecnique to study biofilm production of Listeria monocytogenes isolated in poultry slaughterhouse 
5) Use of conventional and molecular techniques (Real Time PCR) to isolation and quantification of zoonotic bacteria with special interest for Salmonella spp., Listeria monocytogenes and Campylobacter
6) Comparison of different molecular techniques (plasmide profile, rybotiping, RAPD, PFGE, AFLP, MLST) for the genetic characterization of foodborne bacteria
7) Safety criteria identification (POs and FSOs) for egg and egg products, poultry and pork meat
8) Sampling plans definiton to verify the compliance to safety criteria (POs and FSOs) within poultry and swine food chain.
9) Risk evaluation of ready to eat foods: use of innovative treatment to reduce the survival and growth of  pathogen bacteria such as Listeria monocytogenes, E coli VTEC and Salmonella