Foto del docente

Stefania D'Adamo

Junior assistant professor (fixed-term)

Department of Biomedical and Neuromotor Sciences

Academic discipline: BIOS-07/A Biochemistry

Research

My research activity focuses on the study of molecular and cellular mechanisms involved in degenerative processes, with the aim of identifying new therapeutic targets and developing innovative strategies. Specifically, I work on:

1. Non-coding RNA and Post-transcriptional Regulation
  • Investigation of the role of non-coding RNAs (miRNAs, tRFs, isomiRs, etc.) in the modulation of mRNA stability and translation

  • RNA editing

  • Nucleocytoplasmic trafficking and subcellular localization

  • Epigenetic, mitochondrial, and cellular stress-related implications

2. Protein Quality Control and Cellular Stress
  • Mechanisms of proteostasis and protein quality control

  • Signal transduction under stress conditions

  • Analysis of protein aggregation and cellular detoxification systems

3. Metabolism and Cellular Aging
  • Role of nutraceuticals and polyamines in modulating cellular aging

  • Impact on age-related degenerative processes

4. Autophagic Dynamics
  • Studies on regulatory mechanisms of autophagy

  • Dynamics of autophagic flux

  • Role of autophagy in apoptosis, cellular senescence, and disassembly of ribonucleoprotein granules

5. Diseases of Interest

Investigation of cellular and molecular mechanisms in:

  • Osteoarthritis

  • Cardiovascular diseases

Biological Models Used
  • Organoids derived from induced pluripotent stem cells (iPSCs)

  • Primary cell cultures from patients

  • Human and animal cell lines

Technologies and Experimental Approaches
  • Next-Generation Sequencing (NGS), RNA-seq and small RNA-seq

  • Bioinformatic analysis: pathway enrichment, target prediction, RNA–protein and RNA–RNA interaction studies

  • Differentiation of iPSCs into cardiomyocyte-like cells

  • Gene modulation (silencing/overexpression using ASOs, siRNAs, plasmids, mimics)

  • Polysome profiling and RNA/protein co-immunoprecipitation

  • Gene expression analysis (RT-qPCR, Western blot)

  • Enzymatic assays and protein aggregation assessment

  • Fluorescence and 3D confocal microscopy, single-cell analysis in vivo

  • Real-time analysis of cellular metabolism using Seahorse XF and IncuCyte platforms

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