- In vitro and in vivo embryo production in domestic animals
- Cryopreservation of embryos and oocytes in domestic
animals
- Study of mesenchymal stem cells from bone marrow,
adipose tissue, cord blood, fetal fluid and membranes in domestic
animals with particular interest for their microvescicular production and immunophenotype
- Study of Wharton's jelly in domestic animals
- Clinical applications in dog and horse of mesenchymal stem cells
Over the past decade stem cell research has emerged as an area of
major interest for its potential in regenerative medicine
applications. Mesenchymal stem cells will be isolated from equine
and canine adipose tissue, bovine, equine, canine and feline
umbilical cord blood and matrix, fetal fluid and membrane. Isolated
mesenchymal stem cells will be cultured and expanded in vitro;
cells will be differentiated in vitro in ostecyte, condrocyte and
adipocyte and will be characterized with stemness markers. All cell
lines, until third passage, will be cryopreserved. In equine and
dog, stem cells will be used for clinical applications.