The research consists of three main topics:
1) Over-expression of recombinant proteins in Escherichia
coli. The aim of this study is to develop an efficient
expression system in bacteria in order to produce high level of
target proteins.
2) Study of the Mycobacterium tuberculosis protein tyrosin-phosphatase A (MpTpA) and analysis of its conformational transition during catalysis by steady-state and pre-staedy-state assays.
3) Study of human and rabbit muscle lactate dehydrogenase (LDH). In the first case the interest is directed at studying specific inhibitors of the assembly of the tetrameric active enzyme starting from the LDHA monomer. Regarding the rabbit enzyme the research activity is directed to study the effect of trehalose on the activity of the tetrameric enzyme.
