Foto del docente

Alessandra Zambonelli

Full Professor

Department of Agricultural and Food Sciences

Academic discipline: BIO/01 General Botany


Keywords: mycelial pure cultures truffles mycorrhizae rDNA

1 – Studies on the genetic variability inside Tuber spp.  and in particular of T. borchii.  2 – Perfecting new biotechnologies of ectomycorrhizal synthesis using mycelial pure cultures. 3 – Perfecting new morphological and molecular tools for the identification of mycorrhizas. 4 – Studies of the biotic factors (bacteria, genotype of the host plant, etc) and environmental  (light, composition of the substrate, etc) able to induce morphological  modifications on the mycelium of Tuber spp. 5 - Studies on the composition and dynamic of the microbic communities associated to the truffle ascomata. 6. Studies of the medicinal proprieties of the  fungi belonging to the Ganoderna genus.

1 – The genetic variability of the truffles inside Tuber puberulum clade (Tuber borchii, T. maculatum, T. dryophilum, etc) is studied by the analysis of ITS, IGS regions of rDNA and of ß-tubulin and PKC genes. 2 –Numerous strains  of different Tuber spp. were isolated in pure for the fist time (Iotti et al.,, 2002). In vitro and in greenhouse infected plants with T. borchii, T. brumale , T. melanosporum and T. aestivum were obtained. We are improving these mycorrhizal synthesis techniques and we are trying to apply these techniques also to T. magnatum . 3 – Sequencing the ITS regions and given morphological description of the mycorrhizas  different ectomycorrhizal fungi we are providing tools for the identication of ectomycorrhizas in the field. 4 - The aim of this research is to study the effect of different biotic and environmental factors on the morphology of different species of Tuber in the different phases of their ontogenetic life cycle. The research will be carried out in collaboration with the research group of Urbino that will study the molecular mechanisms associated with the observed morphological modifications. In particular we will study the influence of substrate and bacteria on the growth and morphology of the mycelium in vitro. 5 – Microrganisms present in the mycorrhizosphere and associated with the fungal life cycle such as bacteria could influence the mycelial growth of mycorrhizal fungi. In particular Staphylococcus pasteurii, a bacterium isolated by us is able to produce volatile substances with an inhibiting and paramorhogeneus activity on T. borchii (Barbieri et al., 2005). Soil saprobic or ectomycorrizal fungi can favorite or inhibit other ectomycorrhizal fungi in the soil.  The effects of these microrganisms on the growth on Tuber spp. mycelia and their impact on the truffle life cycle are still uninvestigated. We would like to study the fungal and bacterial communities associated to truffle and to investigate their effects on truffle production.6. Studies are in progress on the genetic variability, biochemical characteristics and  antioxidant activity of different strains of Ganoderma spp.

Italian and armenian