Foto del docente

Massimo Guardigli

Associate Professor

Department of Chemistry "Giacomo Ciamician"

Academic discipline: CHIM/01 Analytical Chemistry

Research

Keywords: In situ hybridization/immunohistochemistry Bio-chemiluminescence Time-Resolved Fluorescence (TRF) Imaging Enzyme activity

Study of the photophysical properties of metal complexes, in particular of luminescent lanthanide and transition metal complexes. Conjugation of lanthanide complexes to biospecific reagents (e.g., antibodies) and their use as probes in bioanalytical methods based on time-resolved luminescence measurements.

Development of ultrasensitive luminescence analytical methods for the detection of enzymes, substrates and metabolites. Application to the screening of enzyme inhibitors (e.g., acetylcholinesterase, COX-1 and COX-2 inhibitors) and the measurement of antioxidant activity of biological samples.

Development of ultrasensitive immunoassays based on luminescence detection. Application for the analysis of environmental, biological and clinical samples and for the detection and localization of organic binders in cultural heritages by luminescence imaging microscopy.

Development of luminescence immunohistochemistry and in situ hybridization techniques for the localization of antigens and gene sequences in tissue sections and single cells. Quantitative measurement on target analytes in tissue sections by imaging microscopy.

On-line and off-line coupling of luminescence detection with separative systems, such as Field-Flow Fractionation (FFF) devices, for the study of the fractionation process and the development of new analytical techniques.

Quantitative analysis of drugs and metabolites in biological samples by HPLC with mass spectrometry detection. Application to pharmacokinetics and bioequivalence studies.

Development of portable analytical systems based on bio-chemiluminescence measurements. Development of detection systems bases on CCD sensors and of analytical systems employng microfluidic devices or lateral flow immunoassays.

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