Study of the photophysical properties of metal complexes, in
particular of luminescent lanthanide and transition metal
complexes. Conjugation of lanthanide complexes to biospecific
reagents (e.g., antibodies) and their use as probes in
bioanalytical methods based on time-resolved luminescence
Development of ultrasensitive luminescence analytical methods
for the detection of enzymes, substrates and metabolites.
Application to the screening of enzyme inhibitors (e.g.,
acetylcholinesterase, COX-1 and COX-2 inhibitors) and the
measurement of antioxidant activity of biological samples.
Development of ultrasensitive immunoassays based on luminescence
detection. Application for the analysis of environmental,
biological and clinical samples and for the detection and
localization of organic binders in cultural heritages by
luminescence imaging microscopy.
Development of luminescence immunohistochemistry and in situ
hybridization techniques for the localization of antigens and gene
sequences in tissue sections and single cells. Quantitative
measurement on target analytes in tissue sections by imaging
On-line and off-line coupling of luminescence detection with
separative systems, such as Field-Flow Fractionation (FFF) devices,
for the study of the fractionation process and the development of
new analytical techniques.
Quantitative analysis of drugs and metabolites in biological
samples by HPLC with mass spectrometry detection. Application to
pharmacokinetics and bioequivalence studies.
Development of portable analytical systems based
on bio-chemiluminescence measurements. Development of
detection systems bases on CCD sensors and of analytical
systems employng microfluidic devices or lateral flow