Foto del docente

Massimo Guardigli

Associate Professor

Department of Chemistry "Giacomo Ciamician"

Academic discipline: CHIM/01 Analytical Chemistry

Curriculum vitae

Position

Associate Professor of Analytical Chemistry, University of Bologna

Participant to the Interdepartmental Center for Industrial Research "Renewable Sources, Environment, Sea and Energy" (CIRI-FRAME) and to Interdepartmental Center for Industrial Research "Aerospace" (CIRI-AERO)

 

Education and Training

DSc Degree in Chemistry, University of Bologna: Magna Cum Laude (1989)

PhD Degree in Chemistry, University of Bologna (1994)

Scolarship, FRAE Institute, CNR, Bologna (1994-1995)

Post-doc grant, Institute of Inorganic and Analytical Chemistry, University of Lausanne, Switzerland (1996)

 

University activity

Researcher of Analytical Chemistry, University of Bologna (1998-2001)

Associate Professor of Analytical Chemistry, University of Bologna (2001-)

 

Teaching activity

Analytical Chemistry teaching in graduate courses (Chemistry and Pharmaceutical Technologies, Applied Pharamaceutical Sciences (curriculum Environmental toxicology)

 

Scientific Activity

Study of the photophysical properties of metal complexes, in particular of luminescent lanthanide and transition metal complexes. Conjugation of lanthanide complexes to biospecific reagents (e.g., antibodies) and their use as probes in bioanalytical methods based on time-resolved luminescence measurements.

Development of ultrasensitive luminescence analytical methods for the detection of enzymes, substrates and metabolites. Application to the screening of enzyme inhibitors (e.g., acetylcholinesterase, COX-1 and COX-2 inhibitors) and the measurement of antioxidant activity of biological samples.

Development of ultrasensitive immunoassays based on luminescence detection. Application for the analysis of environmental, biological and clinical samples and for the detection and localization of organic binders in cultural heritages by luminescence imaging microscopy.

Development of luminescence immunohistochemistry and in situ hybridization techniques for the localization of antigens and gene sequences in tissue sections and single cells. Quantitative measurement on target analytes in tissue sections by imaging microscopy.

On-line and off-line coupling of luminescence detection with separative systems, such as Field-Flow Fractionation (FFF) devices, for the study of the fractionation process and the development of new analytical techniques.

Quantitative analysis of drugs and metabolites in biological samples by HPLC with mass spectrometry detection. Application to pharmacokinetics and bioequivalence studies.

 

Personal skills and expertise

Mother tongue: Italian

Reading skills: English (good)

Writing skills: English (good)

Verbal skills: English (fair)

 

 

Technical skills and expertise

Photophysical characterization of luminescent species using steady-state and time-resolved absorption and emission spectroscopic techniques. Development and/or optimization of analytical and bioanalytical methods based on single and coupled enzyme reactions with luminescence detection. Ultrasensitive luminescence imaging techniques applied for the localization of target analytes on macro- and microsamples.

 

Scientific publications

Author of about 90 80 papers on referred journals in the fields of Analytical Chemistry, Physical Chemistry, Inorganic Chemistry.

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