Lorenzo Bertin

NAME:                                   Lorenzo

SURNAME:                           BERTIN

 

DATE OF BIRTH:                  march 12th, 1971

PLACE OF BIRTH:                Bologna (ITALY)

 

ADDRESS (OFFICE):            DICAM, Department of Civil, Environmental and Materials Engineering, Engineering Faculty of the University of Bologna,

via Terracini 28, I- 40131, Bologna (ITALY)

 

TELEPHONE    (OFFICE):    +39 051 2090317

 

E-MAIL:         lorenzo.bertin@unibo.it

 

 

EDUCATION AND CAREER
1) Associate Professor of Chemistry and Biotechnology of Fermentations at the University of Bologna (current position, from September 2014)
2) Assistant Professor of Organic Chemistry at the University of Bologna (from October 2001 to September 2014)
3) Fellowship “Marco Polo” of the University of Bologna for the promotion abroad of young researcher (IV Round, June - December 2003). Title of the Financed project: “Lactato-oxidase and Horseradish-peroxidase immobilization in the development of biosensors for the measurement of l-Lactic acid in FIA (Flow Injection Analysis) systems”. The research was carried out by the Department of Chemical Engineering of the Instituto Superior Tecnico of the Technical University of Lisbon (Portugal), under the supervision of Professor J.M.S. Cabral.
4) Ph.D. in Industrial Chemistry (May 2003), Industrial Chemistry Faculty of the University of Bologna (Italy). Dissertation Title: “Olive mill wastewater treatment and valorisation by using fixed-bed bioreactors”.
5) Master Degree in Chemical Engineering (December 1995, points: 99/100), Engineering Faculty of the University of Bologna (Italy). Dissertation title: “Aerobic biodegradation of polychlorobiphenyls (PCBs) in a fixed-bed bioreactor by a bacterial co-colture immobilised on “Manville” silica beads”

SCIENTIFIC ACTIVITY - KEYWORDS
biowastes valorization; anaerobic digestion; photofermentation; purple baceria; biomethane; biohydrogen; volatile fatty acids; polyhydroxyalkanoates (PHA); polyphenols recovery; solid phase extraction; membranes.

SCIENTIFIC ACTIVITY - BRIEF DESCRIPTION
Lorenzo Bertin's research activity began in the late 1990s at the University of Bologna (Italy), focusing on the biodegradation of priority pollutants in actual-site PCB-contaminated soils by the development of bioprocesses in bioreactors with different nonconventional configurations. The culturing and employment of exogenous specialized bacteria was successfully proposed. Industrial wastewaters contaminated by non-ionic surfactants (polyethoxilated nonylphenols) and sewage sludge collected from secondary treatment plants of industrial wastewaters were then targeted for biodegradation studies, carried out under aerobic and anaerobic conditions, respectively.
The main topic of his Ph.D. project, carried out from 1999 to 2002, was the treatment and valorization of olive mill wastewaters (OMWs), i.e., the aqueous effluent resulting from olive oil production process, in aerobic and anaerobic fixed-bed biofilm reactors. In particular, he isolated two bacterial aerobic strains capable of biodegrading several polyphenolic compounds typically occurring in the wastewaters, which are responsible for the wastewater antimicrobial activity and phytotoxicity. The strains were successfully employed in the physiological condition of immobilized cells in the post-treatment of OMWs fed to anaerobic digestion processes. Anaerobic digestion processes were then investigated by developing an anaerobic biofilm column reactor, which was filled with granular activated carbon and which allowed significantly higher methane productivity with respect to conventional reactors.
OMWs and grape pomaces were then employed as feedstock for the development of multi-purpose biorefinery schemes dedicated to the recovery of polyphenols and the exploitation of the organic leftover for biopolymers (polyhydroxyalkanoates, PHAs) production. Selective, effective, and environmentally compatible solid phase extraction procedures, according to which both the adsorbent and the extraction solvent are recycled into the process, were defined for polyphenol or VFA separation and recovery. For the first time, the selective recovery of OMW polyphenols was coupled to the extensive conversion of the leftover organic matter into volatile fatty acids (VFAs), i.e., the most suitable substrate for aerobic mixed cultures capable of producing PHAs, by developing and optimizing an anaerobic acidogenic biofilm process.
From 2005, he designed, developed, and assessed unconventional anaerobic digestion (AD) processes carried out under methanogenic or dark fermentation conditions. Several organic matrices, among which mechanically sorted-organic fractions of municipal solid waste (MS-OFMSW), sewage sludge and agro-industrial effluents, have been tested as the process feedstock. In particular, he demonstrated that anaerobic microbial consortia capable of efficiently biomethanize polluted MS-OFMSW (recalcitrant to AD) can be obtained according to novel continuous or semicontinuous cultivation protocols, which allow the adaptation of consortia to contaminated organic fractions collected from unsorted MSW. Furthermore, an innovative two-stage AD process, which could combine the advantages of the simplicity of conventional processes with the high efficiency of multistep reactors, was designed and successfully developed. To this aim, the acidogenic stage was directly inserted into the methanogenic vessel according to a concentric design. The production of PHAs from agroindustrial residues has been pursued by the employment of pure bacterial cultures. To this aim, organic agro-industrial biowastes were anaerobically digested under acidogenic conditions, in order to obtain effluent rich in VFAs, which are suitable precursors for the biotechnological production of PHAs. Cupriavidus necator and Pseudomonas putida strains were tested as the main process biocatalysts in the frame of novel process schemes: strains were demonstrated to accumulate PHAs according to a dual-step approach, including a cell growth phase under balanced conditions and a consecutive PHA accumulation phase fed with VFAs. in particular, P. putida was forced to accumulate a specific amorfous and sticky PHA, i.e., polyhydroxyhexanoate (PHH), with peculiar alternative applications with repsect to the ones of conventional PHAs.
Finally, a novel research activity has been developing for the biological removal of sulphide from biogas by photofermentation processeses mediated by purple sulphur bacteria.

MAIN PUBLICATIONS AND BIBLIOMETRIC INDICATORS
1) 68 full papers published in international peer reviewed journals with IF (21 of which published in last 5 years, 2021-2016);
2) 2400 citations and HI = 34 (Scopus database, July 2023);
3) 9 book chapters;
4) 1 national patent.

SCIENTIFIC COORDINATION OF RESEARCH UNITS
1)

2) EU H2020 project ID. 730349 “RES URBIS” (REsources from URban BIo-waSte): scientific cordinator of local unit
3) National PRIN 2017 project "VISION" (Development and promotion of the Levulinic acid and Carboxylate platforms by the formulation of novel and advanced PHA-based biomaterials and their exploitation for 3D printed green-electronics applications): project scientific responsability
4) National PRIN 2012 project “WISE" (Turning organic Waste into Innovative and Sustainable End-products): scientific cordinator of local unit

PARTICIPATION IN EU AND NATIONAL PROJECTS
participation in 10 EU projects (1 Tender, 1 Interreg ADRION, 5 H2020 and 3 FP7 projects); 21 national projects among which 2 AGER projects (grant-making foundation), 13 ministerial projects (7 MIUR-PRIN, 1 MIUR-PON FESR, 2 MIUR-National Technology CLUSTERs and 3 MIPAAF), 1 INAIL, 2 regional POR-FESR and 1 PRIITT funded by Emilia Romagna region

TECHNOLOGY TRANSFER
Co-owner of an Italian patent entitled "Process for the production of carboxylic acids from agroidustrial byproduts, and theri employments" (#102018000008301)
Research activity commissioned by CEFLA S.C. (Imola, Bologna, Italy). Project title: “Set up, assessment and control of anaerobic digestion processes for biomethane production”.
Research activity commissioned by Sadam Engineering (Sadam Eridania S.p.A. group, Bologna, Italy). Project title: “Pre-treatment and anaerobic digestion of stabilized grape pomace”.
Research activity commissioned by Archea Italia s.r.l (Cento, Ferrara, Italy). Project title: “Analyses of biomasses sent to anaerobic digestion processes and of related effluents for bioprocess monitoring”.

AWARDS AND OTHER HONORS
1) Chemical Engineering Journal Top Cited Papers for 2011 and 2012 for the publication: Bertin L., Ferri F., Scoma A., Marchetti L., Fava F. Recovery of high added value natural polyphenols from actual olive mill wastewater through solid phase extraction. Chemical Engineering Journal, 2011, Volume 171, pp. 1287-1293
2) Invited speaker al "Second International Meeting on Environmental Biotechnology and Engineering", Mexico City (Mexico), September 26-29, 2006 (oral contribution entitled “Anaerobic digestion as a process for the decontamination and valorization of activated sludges resulting from plants treating wastewaters contaminated by xenobiotic compounds”)

OTHER TITLES
1) Elected in the Scientific Committee of the National Interuniversitary Consortium “Chemistry for the Environment” (INCA).
2) Member of Organizing Committee of following events of international relevance:
a) "Environmental Microbiology and Biotechnology in the frame of the Knowledge-Based Bio and Green Economy" (EMB2012), International Conference. 10-12 April 2012, Engineering Faculty of the University of Bologna, Bologna (Italy).
b) "Production of fuels, specialty chemicals and biobased products from agro-industrial wastes and surplus", European Summer School. 4-9 September 2006, Engineering Faculty of the University of Bologna, Bologna (Italy).
c) "Innovative approaches to the bioremediation of contaminated sites", European Summer School. 7-11 September 2004, Engineering Faculty of the University of Bologna, Bologna (Italy).
3) Guest Editor for the Special Issue of the “BioMed Research International” journal entitled: “Multipurpose, Integrated 2nd Generation Biorefineries

PUBLICATIONS:

1. Full papers(FP) in peer reviewed journals

FP68) D’Ambrosio V., Martinez G., Jones E., Bertin L., Pastore C. (2023) Ethyl hexanoate rich stream from grape pomace: A viable route to obtain fine chemicals from agro by-products. SEPARATION AND PURIFICATION TECHNOLOGY 309:123100

FP67) Licursi D., Raspolli Galletti A.M., Antonetti C., Martinez G, Jones E., Bertin L., Di Fidio N., Fulignati S., Pasini G., Frigo S. (2022) Tunable Production of Diesel Bio-Blendstock by Rhenium-Catalyzed Hydrogenation of Crude Hexanoic Acid from Grape Pomace Fermentation. Catalysts 12:1550.

FP66) Lanfranchi A., Tassinato G., Valentino F., Martinez G. A., Jones E., Gioia C., Bertin L., Cavinato C. (2022). Hydrodynamic cavitation pre-treatment of urban waste: Integration with acidogenic fermentation, PHAs synthesis and anaerobic digestion processes. CHEMOSPHERE 301: 1-9

FP65) Domingos J. M. B., Martinez G. A., Morselli E., Bandini S., Bertin L. (2022). Reverse osmosis and nanofiltration opportunities to concentrate multicomponent mixtures of volatile fatty acids. SEPARATION AND PURIFICATION TECHNOLOGY 290: 1-10

FP64) Martinez G. A., Puccio S., Domingos J. M. B., Morselli E., Gioia C., Marchese P., Raspolli Galletti A. M., Celli A., Fava F., Bertin L. (2022). Upgrading grape pomace contained ethanol into hexanoic acid, fuel additives and a sticky polyhydroxyalkanoate: an effective alternative to ethanol distillation. Green Chemistry 24: 2882-2892

FP63) Samori' C.; Martinez G.A.; Bertin L.; Pagliano G.; Parodi A.; Torri C.; Galletti P. (2022) PHB into PHB: Recycling of polyhydroxybutyrate by a tandem “thermolytic distillation-microbial fermentation” process. Resources Conservation and recycling 168:1-7

FP62) Duarte M.S.; Sinisgalli E.; Cavaleiro A.J.; Bertin L.; Alves M.M.; Pereira M.A. (2021) Intensification of methane production from waste frying oil in a biogas-lift bioreactor. Renewable Energy 168: 1141-1148

FP61) Degli Esposti, Micaela; Morselli, Davide; Fava, Fabio; Bertin, Lorenzo; Cavani, Fabrizio; Viaggi, Davide; Fabbri, Paola (2021) The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability. FEBS OpenBio 11(4):967-983

FP60) Sarah Notarfrancesco; Elena Morselli; Gonzalo A.Martinez; Weronica Harasimiuk; Joana M.B.Domingos; Andrea Negroni; Fabio Fava; Lorenzo Bertin (2021) Improved recovery of carboxylic acids using sequential cationic-anionic adsorption steps: A highly competitive ion-equilibrium model. Separation and Purification Technology 261: 1-10

FP59) Guneet Kaura, Linsey Garcia-Gonzalez, Kathy Elst, Federica Truzzi, Lorenzo Bertin, Ankita Kaushik, Malini Balakrishnan, Heleen De Wever (2020). Reactive extraction for in-situ carboxylate recovery from mixed culture fermentation. Biochemical Engineering Journal 160:107641

FP58) Marchetti, R., Vasmara, C., Bertin, L., Fiume, F. (2020) Conversion of waste cooking oil into biogas: perspectives and limits. Applied Microbiology and Biotechnology 104(7): 2833-2856

FP57) Nuzzo, A., Puccio, S., Martina, C., Pietrangeli, B., Martinez, G.A., Bertin, L., Mancini, M., Fava, F., Zanaroli, G. (2020) Containment of a genetically modified microorganism by an activated sludge system. New Biotechnology 55:58-64

FP56) Hacifazlioglu M.C.; Tomasini H.R.; Bertin L.; Pek T.O.; Kabay N. (2019) Concentrate reduction in NF and RO desalination systems by membrane-in-series configurations-evaluation of product water for reuse in irrigation. Desalination 466:89-96

FP55) H.R. Tomasini, M.C. Hacifazlioglu, N. Kabay, L. Bertin, T.O. Pek, M. Yuksel (2019). Concentrate management for integrated MBR-RO process for wastewater reclamation and reuse-preliminary tests. J. Water Process Eng. 29:1-7

FP54) M.C. Hacıfazlıoğlu, H.R. Tomasini; N. Kabaya, L. Bertin, T.Ö. Pek, M. Kitiş, N. Yiğit, M. Yüksel (2018). Effect of pressure on desalination of MBR effluents with high salinity by using NF and RO processes for reuse in irrigation. J. Water Process Eng. 25:22-27

FP53) K. De Sitter, L. Garcia-Gonzalez, C. Matassa, L. Bertin, H. De Wever (2018). The use of membrane based reactive extraction for the recovery of carboxylic acids from thin stillage. Separation and Purification Technology 206:177-185

FP52) J.M.B. Domingos, S. Puccio, G.A. Martinez, N. Amaral, M.A.M. Reis, S. Bandini, F. Fava, L. Bertin (2018). Cheese whey integrated valorisation: Production, concentration and exploitation of carboxylic acids for the production of polyhydroxyalkanoates by a fed-batch culture. Chem. Eng. J. 336:47-53

FP51) J.M.B. Domingos, G.A. Martinez, A. Scoma, S. Fraraccio, F.M.Kerckhof, N. Boon, M.A.M. Reis, M.A.M., F. Fava, L. Bertin (2017). Effect of operational parameters in the continuous anaerobic fermentation of cheese whey on titers, yields, productivities, and microbial community structures. ACS Sus. Chem. Eng. 5(2):1400-1407

FP50) D. Pinelli, A.E. Molina Bacca, A. Kaushik, S. Basu, M. Nocentini, L. Bertin, D. Frascari (2016). Batch and Continuous Flow Adsorption of Phenolic Compounds from Olive Mill Wastewater: A Comparison between Nonionic and Ion Exchange Resins. International Journal of Chemical Engineering 2016:ID 9349627

FP49) S. Rebecchi, D. Pinelli, L. Bertin, F. Zama, F. Fava, D. Frascari (2016). Volatile fatty acids recovery from the effluent of an acidogenic digestion process fed with grape pomace by adsorption on ion exchange resins. Chem. Eng. J. 306:629-639

FP48) J.M. Bendada Domingos, G.A. Martinez, A. Scoma, L. Bertin, M.A.M. Reis, F. Fava (2016). Development of a continuous biofilm-based process for the bioconversion of cheese whey into volatile fatty acids. CHEMICAL ENGINEERING TRANSACTIONS 49:193-198

FP47) G. Di Girolamo, M. Grigatti, L. Bertin, C. Ciavatta, L. Barbanti (2016). Enhanced substrate degration and methane yield with maleic acid pre-treatments in biomass crops and residues. BIOMASS & BIOENERGY 54:182-190

FP46) S.I. Shayan, F.A. Agblevor, L. Bertin, R.C. Sims (2016). Hydraulic retention time effects on wastewater nutrient removal and bioproduct production via rotating algal biofilm reactor. BIORES. TECHNOL. 211:527-533

FP45) D. Frascari, A.E. Molina Bacca, F. Zama, L. Bertin, F. Fava, D. Pinelli (2016). Olive mill wastewater valorisation through phenolic compounds adsorption in a continuous flow column. CHEM. ENG. J. 283:293-303

FP44) A. Scoma, F. Varela-Corredor, L. Bertin, C. Gostoli, S. Bandini (2016). Recovery of VFAs from anaerobic digestion of dephenolized Olive Mill Wastewaters by Electrodialysis. SEPARATION AND PURIFICATION TECHNOLOGY 159:81-91

FP43) G.A. Martinez, S. Rebecchi, D. Decorti, J.M.B. Domingos, A. Natolino, D. Del Rio, L. Bertin*, C. Da Porto, F. Fava (2016) Towards multi-purpose biorefinery platforms for the valorisation of red grape pomace: production of polyphenols, volatile fatty acids, polyhydroxyalkanoates and biogas. GREEN CHEM. 216:261-270

FP42) A. Scoma, S. Rebecchi, L. Bertin*, F. Fava (2016) High impact biowastes from South European agro-industries as feedstock for second-generation biorefineries. CRIT. REV. BIOTECHNOL. 36:175-189

FP41) G.A. Martinez, L. Bertin*, A. Scoma, S. Rebecchi, G. Braunegg, F. Fava (2015) Production of polyhydroxyalkanoates from dephenolised and fermented olive mill wastewaters by employing a pure culture of Cupriavidus necator. BIOCHEM. ENG. J. 97:92-100

FP40) M. Monti, A. Scoma, G. Martinez, L. Bertin*, F. Fava (2015) Uncoupled hydrogen and volatile fatty acids generation in a two-step biotechnological anaerobic process fed with actual site wastewater. NEW BITECHNOL. 32:341-346

FP39) G. Di Girolamo, L. Bertin, L. Capecchi, C. Ciavatta, L. Barbanti (2014) Mild alkaline pre-treatments loosen fibre structure enhancing methane production from biomass crops and residues. BIOMASS & BIOENERGY 71:318-329

FP38) A. Scoma, L. Durante, L. Bertin, F. Fava (2014) Acclimation to hypoxia in Chlamydomonas reinhardtii: can biophotolysis be the major trigger for long-term H2 production? NEW PHYTOL. 204(4):890-900

FP37) S. Campanari, F.A. Silva, L. Bertin, M. Villano, M. Majone (2014) Effect of the organic loading rate on the production of polyhydroxyalkanoates in a multi-stage process aimed at the valorization of olive mill wastewaters. Int. J. Biol. Macromol. 71:34-41

FP36) L. Barbanti, G. Di Girolamo, M. Grigatti, L. Bertin, C. Ciavatta (2014) Anaerobic digestion of annual and multi-annual biomass crops. Industrial Crops and Products 56:137-144

FP35) L. Martino, V. Cruz, A. Scoma, F. Freitas, L. Bertin, M. Scandola, M.A.M. Reis (2014) Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil. Int. J. Biol. Macromol. 71:117-123

FP34) L. Sciubba, L. Bertin, D. Todaro, C. Bettini, F. Fava, D. Di Gioia (2014) Biodegradation of low-ethoxylated nonylphenols in a bioreactor packed with a new ceramic support (Vukopor ® S10). Environ. Sci. Pollut. Res. 21:3241–3253

FP33) A.El Mekawy, L. Diels, H. De Wever, L. Bertin, D. Pant (2014) Potential biovalorization techniques for olive mill biorefinery wastewater. Biofuels, Bioprod. Bioref. 8(2):283-293

FP32) A. Scoma, L. Bertin*, F. Fava (2013) Effect of hydraulic retention time on biohydrogen and volatile fatty acids production during acidogenic digestion of dephenolized olive mill wastewaters. BIOMASS & BIOENERGY 48:51-58

FP31) L. Bertin*, S. Grilli, A. Spagni, F. Fava (2013) Innovative two-stage anaerobic process for effective codigestion of cheese whey and cattle manure. BIORES. TECHNOL. 128:779-783

FP30) F. Puoci, A. Scoma, G. Cirillo, L. Bertin, F. Fava, N. Picci (2012). Selective extraction and purification of gallic acid from actual site olive mill wastewaters by means of molecularly imprinted microparticles. CHEM. ENG. J. 198-199:529–535

FP29) A. Scoma, C. Pintucci, L. Bertin*, P. Carlozzi, F. Fava (2012). Increasing the large scale feasibility of a solid phase extraction procedure for the recovery of natural antioxidants from olive mill wastewaters. CHEM. ENG. J. 198-199:103-109

FP28) A. Scoma, L. Bertin, C. Pintucci, S. Raddi, F. Fava (2012). Inhibition of photosystem 2 in starch-enriched Chlamydomonas reinhardtii cells prevents the efficient induction of H2 production in sulfur-depleted cultures. International Journal of Hydrogen Energy 37(14):10604–10610

FP27) M. Cappelletti, G. Bucchi, J. De Sousa Mendes, A. Alberini, S. Fedi, L. Bertin, D. Frascari (2012). Biohydrogen production from glucose, molasses and cheese whey by sospended and attached cells of four hyperthermophilic Thermotoga strains. J. CHEM. TECHNOL. BIOTECHNOL. 87(9):1291-1301

FP26) L. Bertin*, C. Bettini, G. Zanaroli, S. Fraraccio, A. Negroni, F. Fava (2012). Acclimation of an anaerobic consortium capable of an effective biomethanization of mechanically-sorted organic fraction of municipal solid waste through a semi-continuous enrichment procedure. J. CHEM. TECHNOL. BIOTECHNOL. 87(9):1312-1319

FP25) S. Casali, M. Gungormusler, L. Bertin, F. Fava, N. Azbar (2012). Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol. BIOCHEM. ENG. J. 64:84-90

FP24) L. Bertin*, C. Bettini, G. Zanaroli, D. Frascari, F. Fava (2012). A continuous-flow approach for the development of an anaerobic consortium capable of an effective biomethanization of a mechanically sorted-organic fraction of municipal solid waste as the sole substrate. WATER RES. 46:413-424

FP23) A. Scoma, L. Bertin*, G. Zanaroli, S. Fraraccio, F. Fava (2011). A physicochemical biotechnological approach for an integrated valorization of olive mill wastewater. Biores. Technol. 102:10273-10279

FP22) L. Bertin*, F. Ferri, A. Scoma, L. Marchetti, F. Fava. (2011). Recovery of high added value natural polyphenols from actual olive mill wastewater through solid phase extraction. CHEM. ENG. J. 171:1287-1293

FP21) F. Ferri, L. Bertin*, A. Scoma, L. Marchetti, F. Fava (2011). Recovery of low molecular weight phenols through solid-phase extraction. CHEM. ENG. J. 166:994-1001

FP20) L. Bertin*, S. Capodicasa, S. Fedi, D. Zannoni, L. Marchetti, F. Fava. (2011). Biotransformation of a highly-chlorinated PCB mixture in an activated sludge collected from a Membrane Biological Reactor (MBR) subjected to anaerobic digestion. J. HAZ. MAT. 186:2060-2067

FP19) L. Bertin*, S. Lampis, D. Todaro, A. Scoma, G. Vallini, L. Marchetti, M. Majone, F. Fava. (2010) Anaerobic acidogenic digestion of olive mill wastewaters in biofilm reactors packed with ceramic filters or granular activated carbon. WATER RES. 44(15):4537-4549

FP18) M. Beccari, L. Bertin, D. Dionisi, F. Fava, S. Lampis, M. Majone, F. Valentino, G. Vallini, M. Villano. (2009). Exlopiting olive mill effluents as a renewable resource for production of biodegradable polymenrs through a combined anaerobic-aerobic process. J. CHEM. TECHNOL. BIOTECHNOL. 84 (17):901-908

FP17) D. Di Gioia, L. Sciubba, L. Bertin, C. Barberio, L. Salvadori, S. Frassinetti, F. Fava. (2009). Nonylphenol polyethoxylate degradation in aqueous waste by the use of batch and continuous biofilm bioreactors. WATER RES. 43:2977-2988

FP16) L. Bertin*, D. Todaro, C. Bettini, F. Fava (2008). Anaerobic codigestion of the mechanically sorted organic fraction of a municipal solid waste with cattle manure in packed microcosms under batch conditions. WATER SCI. TECHNOL. 58(9):1735-1742

FP15) L. Bertin, S. Capodicasa, F. Occulti, S. Girotti, L. Marchetti, F. Fava (2007). Microbial processes associated to the decontamination and detoxification of a polluted activated sludge during its anaerobic stabilization. WATER RES. 41:2407-2416

FP14) L. Bertin*, D. Di Gioia, C. Barberio, L. Salvadori, L. Marchetti, F. Fava (2007) Biodegradation of polyethoxylated nonylphenols in packed-bed biofilm reactors. Ind. Eng. Chem. Res. 46:6681-6687

FP13) L. Bertin, M.C. Colao, M. Ruzzi, L. Marchetti, F. Fava (2006). Performances and microbial features of an aerobic packed-bed biofilm reactor developed to post-treat an olive mill effluent from an anaerobic GAC reactor. MICROBIAL CELL FACTORIES 5:16

FP12) D. Dionisi, L. Bertin, L. Bornaroni, S. Capodicasa, M. Petrangeli Papini, F. Fava (2006). Removal of organic xenobiotics in activated sludges under aerobic conditions and anaerobic digestion of the adsorbed species. J. Chem. Technol. Biotechnol. 81(9):1496-1505

FP11) V. Vojinovic, L. Bertin, J.M.S. Cabral, L. Fonseca (2006). Horseradish peroxidise combined with oxidase enzymes a valuable bioanalytical tool: lactate oxidase – a case study. ENGINEERING IN LIFE SCIENCES 6:181-186

FP10) D. Di Gioia, L. Bertin, G. Zanaroli, L. Marchetti, F. Fava (2006). Polychlorinated biphenyl degradation in aqueous wastes by employing continuous fixed-bed bioreactors. Process Biochem. 41:935-940

FP09) L. Bertin, S. Berselli, F. Fava, M. Petrangeli-Papini, L. Marchetti (2004). Anaerobic digestion of olive mill wastewater s in biofilm reactors packed with granular activated carbon and “Manville” silica beads. WATER RES. 38:3167-3178

FP08) L.Bertin, M.C. Colao, M. Ruzzi, F. Fava (2004). Performances and microbial features of a granular activated carbon packed-bed biofilm reactor capable of an efficient anaerobic digestion of olive mill wastewaters. FEMS MICROBIOL. ECOL. 48:413-423

FP07) F. Fava, L. Bertin, S. Fedi, D. Zannoni (2003). Methyl-beta-cyclodextrin-enhanced solubilization and aerobic biodegradation of polychlorinated biphenyls in two aged-contaminated soils. BIOTECHNOL. BIOENG. 81:381-390

FP06) D. Di Gioia, L. Bertin, F. Fava, L. Marchetti (2001). Biodegradation of hydroxylated and methoxylated benzoic, phenilacetic and phenilpropenoic acids present in olive mill wastewaters by two bacterial strains. RES. MICROBIOL. 152:83-93

FP05) D. Di Gioia, F. Fava, L. Bertin, L. Marchetti (2001) Biodegradation of synthetic and naturally occurring mixtures of mono-cyclic aromatic compounds present in olive mill wastewaters by two aerobic bacteria. APPL. MICROBIOL. BIOTECHNOL. 55:619-626

FP04) L. Bertin, M. Majone, D. Di Gioia, F. Fava (2001). An aerobic fixed-phase biofilm reactor system for the degradation of the low-molecular weight aromatic compounds occurring in the effluents of anaerobic digestors treating olive mill wastewaters. J. BIOTECHNOL. 87:161-177

FP03) F. Fava, L. Bertin, D. Pinelli, M. Nocentini (1999). Characterisation of the indigenous bacteria involved in the ex-situ aerobic bioremediation of a polycyclic aromatic hydrocarbon-contaminated soil. ANNALI DI CHIMICA 89:777-782

FP02) F. Fava, L. Bertin, L. Marchetti (1999) Influence of the exogenous carbon source on the ex-situ bioremediation of a chronically PCB-contaminated soil. ANNALI DI CHIMICA 89: 771-776

FP01) F. Fava, L. Bertin (1999) Use of exogenous specialised bacteria in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in slurry phase conditions. BIOTECHNOL. BIOENG. 64:240-249

2. Book Chapters (BC)

BC9) Kabay N., Haclfazlloǧlu M.C., Parlar A., Tomasini H.R., Falizi N.J., Gundogdu M., Jarma Y.A., Pek T.Ö., Bertin L., Yüksel M. (2022). Post-treatment of MBR effluent by pressure-driven membrane processes for agricultural irrigation. In: World Scientific Reference Of Water Science, Volume 3-3, Pages 75 - 104

BC8) Kabay N., Haclfazlloǧlu M.C., Parlar A., Tomasini H.R., Falizi N.J., Pek T.Ö., Bertin L., Yüksel M. (2021) Application of Reverse Osmosis (RO) and Nanofiltration (NF) Processes for Desalination and Reuse of Membrane Bioreactor (MBR) Effluent as Irrigation and Process Water. In: Materials and Energy Volume 17, Pages 81 – 127, World Scientific, DOI:10.1142/9789811226984_0003

BC7) M. Majone, F. Valentino, L. Bertin, S. Rossetti, G. Zanaroli, G. Martinez, A. Nuzzo, S. Puccio e F. Fava (2017). Bioprocessi per la produzione di polimeri biodegradabili. In: Bioprocessi Innovativi per la Valorizzazione di Rifiuti Organici - salute e sicurezza nelle biotecnologie industriali. Progetto inail Bric 2015; collana “Salute e Sicurezza”, INAIL 2017. ISBN 978-88-7484-584-2

BC6) L. Bertin, D. Frascari, H. Domínguez, E. Falqué, F.A. Riera Rodriguez, S.A. Blanco (2015). Conventional purification and isolation. In: Galanakis C., Food Waste Recovery: Processing Technologies and Industrial Techniques. p. 149-172, San Diego:Elsevier Inc., ISBN: 978-0-12-800351-0

BC5) R. Verdini, M. Petrangeli Papini, M. Majone, I. Di Somma, D. Spasiano, R. Marotta, R. Andreozzi, L. Bertin, F. Fava, D. Frascari, D. Pinelli, M. Nocentini, S. Milia, A.Carucci, F. Aulenta, S. Rossetti. (2013). Processi innovativi per il risanamento sostenibile di acque sotterranee contaminate da composti clorurati. In: F. Cecchi, M. Majone, G. Mancini. Xenobiotici e contaminanti emergenti – L'analisi, il controllo ed il trattamento nelle acque reflue civili, industriali e di falda. (Cap. 9, pp. 1-65). Marghera (VE, Italy): INCA (Interuniv. Cons. Chem. for the Environ.). ISBN: 88-88214-02-X.

BC4) F. Valentino, M. Villano, L. Bertin, M. Beccari, M. Majone. (2011). Olive Oil Wastewater as a Renewable Resource for Production of Polyhydroxyalkanoates. In: V. Mittal. Renewable Polymers: Synthesis, Processing, and Technology. (pp. 175 - 219). HOBOKEN, NJ: John Wiley & Sons, Inc. ISBN: 9780470938775

BC3) F. Fava, L. Bertin. (2008). Intensified processes for methane production from olive mill wastewaters. In: F. Fava, P. Canepa. European Summer School "Production of fuels, specialty chemicals and biobased products from agro-industrial wastes and surplus". (pp. 129-142). Marghera (VE, Italy): INCA (Interuniv. Cons. Chem. for the Environ.). ISBN: 88-88214-18-6

BC2) F. Fava, D. Di Gioia, L. Bertin, S. Berselli, G. Zanaroli, L. Marchetti, D. Zannoni, M. Molnár, K..Gruiz, É. Fenyvesi, J. Szejtli. (2005). Biogenic agents for improving the bioavailability and biodegradation of hydrophobic pollutants in soils. In: F. Fava, P. Canepa. Innovative approaches to the bioremediation of contaminated sites. (pp. 267-278). Marghera (VE, Italy): INCA (Interuniv. Cons. Chem. for the Environ.). ISBN: 88-88214-33-X

BC1) F. Fava, L. Bertin, L. Marchetti (2002) Studio di biotrattabilità del suolo ACNA A5136B. In: Soil Remediation Series N. 1. Progetto SISIFO. Risultati 1° anno di attività (pp. 73-80).. A cura di P. Canepa. Marghera (VE): Consorzio Interuniversitario Nazionale “La Chimica per l'Ambiente” (INCA). ISBN: 88-88214-02-X

3. Extended Abstracts (EA) on conference proceeding

EA32) A. Scoma, F. Fava, L. Bertin (2012). A multi-step physicochemical-biotechnological approach for the valorization of olive mill wastewaters. Proceedings of the “WasteEng12” - 4th International Conference on Engineering for Waste and Biomass Valorization, Porto (Portugal), September 10 – 13, 2012. (pp. 509 - 514) Ed: Ecole des Mines d'Albi, Albi (France). ISBN: 9791091526005

EA31) J. De Sousa Mendes, A. Alberini, G. Bucchi, C. Manfreda, M. Cappelletti, D. Pinelli, S. Fedi, L. Bertin, D. Frascari. Prove batch per la produzione di idrogeno tramite digestione anaerobica di scarti dell'industria alimentare utilizzando quattro ceppi batterici termofili del genere Thermotoga. Convegno GRICU 2012 - Ingegneria Chimica: dalla nanoscala alla macroscala. (pp. 655 – 658). Montesilvano Lido (PE), 16-19 Settembre 2012.

EA30) M. Grigatti, L. Barbanti, G. Di Girolamo, L. Cavani, L. Bertin, C. Ciavatta (2012). Anaerobic digestion of alternative energy crops. Proceedings “Venice 2012” del “Fourth International Symposium on Energy from Biomass and Waste”, San Servolo, Venezia (Italy), 12-15 Novembre 2012. (pp. 1 - 9). CISA Publisher (Padova, Italy). ISBN: 9788862650069

EA29) M. Majone, R. Andreozzi, F. Aulenta, L.Bertin, A. Carucci, D. Frascari, R. Marotta, S. Milia, S. Rossetti, R. Verdini (2012). Processi innovativi per il risanamento sostenibile di acque sotterranee contaminate da composti clorurati (Progetto PRIN 2008). A cura di L. Morselli, ECOMONDO 2012. Rimini. 07-10/11/2012. Sant'Arcangelo di Romagna (RN): Maggioli Editore

EA28) L. Bertin, C. Bettini, G. Zanaroli, S. Fraraccio, A. Negroni, F. Fava (2011). Repeated batch approach as a feasible procedure for the acclimatization of anaerobic consortia capable of an effective biomethanization of mechanically-sorted organic fraction of municipal solid waste. A cura di F. Fava e N. Kalogerakis, Proceedings of the 5th European Bioremediation Conference, 4-7/07/2011, Chania, Crete, Greece.

EA27) A. Scoma, L. Bertin, G. Zanaroli, S. Fraraccio, F. Fava (2011). A chemical-biological integrated approach for the valorization of olive mill wastewaters. A cura di F. Fava e N. Kalogerakis, Proceedings of the 5th European Bioremediation Conference, 4-7/07/2011, Chania, Crete, Greece.

EA26) L. Bertin, F. Ferri, L. Marchetti, F. Fava (2010). Recovery of olive mill wastewater polyphenols through liquid-solid extraction. Proceedings of the 2nd International Conference of IAMAW, 17-19/06/2011, Izmir, Turkey

EA25) L. Bertin, M. Villano, M. Majone, M. Beccari, C. Bettini, F. Fava. (2009). Biotech production of polyhydroxyalcanoates from olive mill wastewater. A cura di L. Morselli, Ecodesign per il pianeta - Soluzioni per un ambiente pulito e per una nuova economia. Atti dei seminari. ECOMONDO 2009. Rimini. 28-31/10/2009. (pp. 399 - 405). ISBN: 978-88-387-5360-1. Sant'Arcangelo di Romagna (RN): Maggioli Editore

EA24) D. Di Gioia, L. Sciubba, L. Bertin, F. Fava (2008). Non ionic surfactant biodegradation by a microbial consortium obtained from textile wastewaters in fixed-bed bioreactors. E-proceedings of the 8th AUTEX Conference, 24-26/06/2008, Biella, Italy. (pp. 1 - 8). ISBN: 978-88-89280-49-2

EA23) L. Bertin, D. Todaro, F. Fava. (2008). Anaerobic digestion of the mechanically sorted organic fraction of a municipal solid waste and its co-digestion with manure in packed-bed biofilm systems under batch conditions. A cura di M. Hamdi, E-Proceedings of the VTH ISAD-SW&EC (International Symposium on Anaerobic Digestion of Solid Wastes and Energy Crops), 24-28/05/2008, Hammamet (Tunisia). (pp. 7A5 - 1 - 7A5 - 7). LONDON: IWA

EA22) M. Beccari, L. Bertin, D. Dionisi, F. Fava, M. Majone, M. Villano (2008). Exploiting olive oil mill effluents as a renewable resource for production of biodegradable polymers through an anaerobic-aerobic process. A cura di N. Kalogerakis, F. Fava e S.A. Banwart, E-Proceedings of the 4th European Bioremediation Conference, 3-6/09/2008, Chania, Greece. (pp. ID 154 p.1 - ID 154 p.4). ISBN: 978-960-8475-12-0

EA21) D.Dionisi, L. Bertin, S. Capodicasa, F.Fava, M. Majone. (2007). Olive mill wastewaters anaerobic digestion in mesophilic and thermophilic batch packed-bed biofilm reactors for the accumulation of volatile fatty acids as monomers employed in the biological production of polyhydroxyalkanoates. Proceedings book of Italic4 - Science and Technology of Biomasses: Advances and Challenges. Materials, Chemicals and Processes from Agricultural and Forest Biomass, 8-10/05/2007, Monte Porzio Catone, Rome, Italy. (pp. 155 - 158)

EA20) L. Bertin, D. Dionisi, D. Todaro, M. Villano, F. Fava, M. Majone. (2007). Valorization of olive mill wastewaters as a renewable resource for the biological production of polyhydroxyalkanoates. E-proceedings of the international conference: New Technologies for the treatment and valorization of agro by-products, 3-5/10/2007, ISRIM, Terni

EA19) L. Bertin, M.C. Colao, M. Ruzzi, F.Fava. (2007). Biomethanization and decontamination of olive mill wastewaters through an integrated anaerobic-aerobic biofilm process. E-proceedings of the international conference: New Technologies for the treatment and valorization of agro by-products, 3-5/10/2007, ISRIM, Terni

EA18) L. Sciubba, D. Todaro, D. Di Gioia, L. Bertin, F. Fava (2007). Degradazione di nonilfenoli polietossilati in un bireattore aerobico impaccato con un supporto ceramico innovativo. A cura di L. Morselli, La Cultura e le tecnologie ambientali in Italia ed in Europa – Gestione e nuove tecnologie di trattamento della acque e dei fanghi di depurazione, gestione e bonifica dei siti contaminati, emissioni industriali ed immissioni: i nuovi problemi posti dalle particelle ultrafini, monitoraggio e biomonitoraggio. Atti dei seminari. ECOMONDO 2007. 7-10/11/2007, Rimini. (vol. 2, pp. 131 - 136). ISBN: 978-88-387-3982-X. Milano: Maggioli Editori

EA17) D. Todaro, F. Occulti, S. Berselli, G. Camera Roda, L. Bertin, L. Marchetti F. Fava (2007). Sviluppo di tecnologie innovative per la bonifica di suoli contaminati da policlorobifenili (PCB). A cura di L. Morselli, La Cultura e le tecnologie ambientali in Italia ed in Europa – Gestione dei rifiuti, compost e trattamento biologico, sostenibilità del territorio e certificazione ambientale. Atti dei seminari. ECOMONDO 2007. 7-10/11/2007, Rimini. (vol. 1, pp. 522 - 527). ISBN: 978-88-387-3979-X. Milano: Maggioli Editori

EA16) F. Fava, S. Capodicasa, L. Bertin (2006). Degradazione di microinquinanti organici nel processo di digestione anaerobica di fanghi contaminati. A cura di L. Morselli, Ambiente: tecnologie, controlli e certificazioni per il recupero e la valorizzazione di materiali ed energie. Gestione delle acque, gestione e bonifica dei siti inquinati, qualità dell'aria, fonti rinnovabili ed efficienza energetica, telerilevamento e GIS. Atti dei seminari. ECOMONDO 2006. 8-11/11/2006, Rimini. (vol. 2, pp. 24 - 28). ISBN: 88.387.3687.1. Milano: Maggioli Editori

EA15) L. Bertin, D. Di Gioia, C. Barberio, L. Salvadori, F. Fava. (2006). Packed-bed biofilm reactors employed in the bioremediation of aqueous wastes contaminated by ethoxylate nonylphenols. A cura di F. Cecchi, Proceedings of First Mediterranean Congress Chemical Engineering for Environment (1stMCCEE), 4-6/10/2006, San Servolo (Venice), Italy. (pp. 345 - 353)

EA14) L. Bertin, M.C. Colao, M. Ruzzi, L. Marchetti, F. Fava. (2006). Performances and microbial features of an integrated anaerobic-aerobic biofilm process for the biogasification and decontamination of olive mill wastewaters. E-proceedings of the Second International Meeting on Environmental Biotechnology and Engineering (2IMEBE), 26-29/09/2006, Mexico City (Mexico). (pp. 95/1 - 95/7). ISBN: 970-95106-0-6

EA13) L. Bertin, S. Capodicasa, F. Occulti, F. Fava (2006). Anaerobic digestion as a process for the decontamination and valorization of activated sludges resulting from plants treating wastewaters contaminated by xenobiotic comopunds. E-proceedings of the Second International Meeting on Environmental Biotechnology and Engineering (2IMEBE), 26-29/09/2006, Mexico City (Mexico). (pp. 133/1 - 133/8). ISBN: 970-95106-0-6

EA12) D. Di Gioia, L. Bertin, L. Salvadori, C. Barberio, F. Fava (2005). Sviluppo di reattori a cellule immobilizzate per il trattamento di acque reflue contaminate da nonilfenoli polietossilati. A cura di L. Morselli, Tecnologie innovative per l'industrializzazione del sistema ambiente: acqua, siti contaminati, atmosfera, politiche energetiche, certificazioni ambientali, organismi geneticamente modificati. Atti dei seminari. Ecomondo 2005. 26-29/10/2005, Rimini. (vol. 2, pp. 170 - 176). ISBN: 88.387.3433.X. Milano: Maggioli Editori

EA11) D. Dionisi, L. Bertin, L. Bornoroni, S. Capodicasa, M. Petrangeli Papini, F. Fava (2005). Fate of xenobiotic pollutants occurring in a sewage sludge under aerobic and anaerobic conditions. A cura di L. Morselli, Tecnologie innovative per l'industrializzazione del sistema ambiente: acqua, siti contaminati, atmosfera, politiche energetiche, certificazioni ambientali, organismi geneticamente modificati. Atti dei seminari. Ecomondo 2005. 26-29/10/2005, Rimini. (vol. 2, pp. 99 - 104). ISBN: 88.387.3221.3. Milano: Maggioli Editori

EA10) L. Bertin, M.C. Colao, M. Ruzzi, L. Marchetti, F. Fava (2005). Use of packed-bed bioreactors in an anaerobic-aerobic integrated process for the treatment and the valorization of olive mill wastewater. E-Proceedings of the Congress: WATMED 2 - Water Resources in Mediterranean Basin. 14-17/11/2005, Marrakech (Morocco)

EA09) M.C. Colao, M. Ruzzi, L. Bertin, F. Fava (2004). Molecular microbial diversity and technological features of a packed-bed biofilm digestor capable of olive mill wastewater anaerobic digestion and valorisation. European symposium on Environmental Biotechnology (ESEB 2004), 25-28/04/2004, Oostende, Belgium. (pp. 377 - 382). ISBN: 90 5809 653 X. Ghent: W. Verstraete

EA08) F. Fava, G. Zanaroli, L. Bertin, D. Di Gioia, L. Marchetti. (2004). Effects of an industrial mixture of methylated-b-cyclodextrins (RAMEB) in the bioremediation of artificially and aged PCB-contaminated soils. Volume II degli atti del convegno GRICU 2004: Nuove Frontiere di Applicazione delle Metodologie dell'Ingegneria Chimica, 12-15/09/2004, Ischia (NA). (pp. 737 - 740). ISBN: 88 87030 80 4

EA07) L. Bertin, F. Fava, M.C. Colao, M. Ruzzi, M. Majone. (2004). Olive mill wastewaters treatment and valorisation in biofilm reactors packed with granular activated carbon or silica beads. Volume II degli atti del convegno GRICU 2004: Nuove Frontiere di Applicazione delle Metodologie dell'Ingegneria Chimica, 12-15/09/2004, Ischia (NA). (pp. 881 - 884). ISBN: 88 87030 80 4

EA06) L delle Metodologie dell'Ingegneria Chimica. Bertin, L. Fonseca, J.M.S. Cabral. (2004). Sviluppo di un biosensore enzimatico ad enzimi immobilizzati per la misura di acido l-Lattico in sistemi FIA (Flow Injection Analysis). Volume I degli atti del convegno GRICU 2004: Nuove Frontiere di Applicazione delle Metodologie dell'Ingegneria Chimica, 12-15/09/2004, Ischia (NA). (pp. 385 - 388). ISBN: 88 87030 80 4

EA05) L. Bertin, F. Fava. (2004). Olive mill wastewater valorization by using passively immobilized anaerobic biomass. COST 840 expert meeting: Applications of Immobilization/Bioencapsulation in Medicine, Pharmacy, Food Technology and Biotechnology. 25-27/06/2004, Belgrado (Serbia), CHEMICAL INDUSTRY 58:95-98

EA04) F. Fava, L. Bertin, D. Di Gioia, G. Zanaroli, L. Marchetti (2003). Methylated-ß-cyclodextrins enhance the bioavailability and aerobic degradation of polychlorinated biphenyls in soils. Proceedings of the Second European Bioremediation Conference, 30/06-04/07/2003, a cura di N. Kalogerakis, Chania, Crete, Greece. (pp. 139-142)

EA03) F. Fava, L. Bertin, M.C. Colao, S. Berselli, M. Majone, M. Ruzzi (2003). Use of packed-bed biofilm reactors in the anaerobic digestion of olive mill wastwater. Proceedings of the Second European Bioremediation Conference, 30/06-04/07/2003, a cura di N. Kalogerakis, Chania, Crete, Greece. (pp. 523-526)

EA02) F. Fava, L. Bertin, D. Di Gioia, G. Zanaroli, L. Marchetti (2003) Use of methylated- ß-cyclodextrins in the aerobic biodegradation of PCB-contaminated soils. In: I Quaderni di Ecomondo – Atti del Seminario. Le bonifiche dei siti contaminati. Aspetti normative, tecnologici e di controllo. A cura di L. Morselli, Collana Ambiente e Territorio, Rimini. (Vol. 3, pp. 162-171). Repubblica di San Marino: Maggioli Editori. ISBN: 88.387.3106.3

EA01) L. Bertin, M.C. Colao, M. Ruzzi, M. Majone, F. Fava (2003). A granular activated carbon (GAC) packed-bed reactor for a more efficient anaerobic treatment and valorization (via methane production) of olive mill wastewaters. Proceedings of the International Workshop Mediterranean Reuse of Water, December 5th, 2003, a cura di Massimiliano fenice, Viterbo, Italy. (pp. 25-32)