Aim of the reserch activity performed in the laboratory "PRISM:
Physiological Regulation in Sleeping Mice" at Department of
Biomedical and Neuromotor Sciences (DIBINEM), Physiology Unit, is
the study of regulatory processes during the different states
of wake-sleep cycle in genetically engenered mice.
Any given change in genetic information typically entails an array
of differences in interacting physiologic functions, much as a beam
of light may be dispersed in its component colours, Physiologic
functions such as cardiovascular and respiratory control differ
widely between wake-sleep behaviours. Mice are the mammalian
species of choice for studies on functional genomics.
The PRISM lab investigates cardiorespiratory phenotype as a
function of the wake-sleep behaviour in mice harbouring changes in
genetic information, with a focus on genetically-engineered mouse
models of human diseases. The main goal of this research is to help
unravel the central neural and genetic mechanisms that underlie the
physiologic and pathologic effects of sleep on cardiorespiratory
control.
Research activity at the PRISM lab is presently focused on the
study of hypothalamic integrative activity during sleep. The
hypothalamus is a key region in the control of diverse physiologic
functions, such as thermoregulation, osmoregulation, metabolism,
and autonomic cardiovascular control. The hypothalamic networks
underlying these functions are tightly entangled and share common
substrates with the regulation of wake-sleep states. Mouse strains
with genetic impairments of specific signalling molecules are a
powerful tool to understand the role of specific neurotransmitters,
neuromodulators, and neural pathways in integrative hypothalamic
function during sleep.
The key technical asset of the PRISM lab is to perform simultaneous
long-term recordings of multiple physiologic signals in
freely-behaving mice. Blood pressure and heart rate are measured by
telemetry, which is the technique at the state of the art in
conscious mice. These measurements are synchronized with
electroencephalographic and electromyographic (postural muscle
tone) signals acquired with conventional cable transmission.
Respiratory activity is monitored non-invasively with the technique
of whole-body plethysmography, which is coupled to measurement of
diaphragm electromyography to discriminate central from obstructive
apnoeas. Stereotaxic implantation of intracerebral microcannulas
allows selective pharmacological manipulation of hypothalamic
circuitry. The PRISM lab is also equipped for development and
maintenance of mouse colonies. Mouse genotyping with qualitative
PCR, gene expression analysis with real-time quantitative PCR, and
immunoenzymatic/immunofluorescent assays on plasma and tissue
samples are performed with state-of-the-art molecular biology
equipment in collaboration with the Centre for Applied Biomedical
Research – CRBA at the Bologna University Hospital. Finally,
researchers at the PRISM lab are actively involved in translating
mathematic tools for analysis and modelling of biosignals developed
in the lab to the clinical setting by means of collaborative
studies on human subjects.
Technical Capabilities and areas of
expertise
EEG/EMG measurements in mice
Sleep deprivation (gentle handling) in mice
Sleep scoring and analysis in mice
Telemetric measurement of blood pressure and heart rate in
mice
Measurement of respiratory function with whole-body plethysmography
and diaphragmatic EMG in mice
Intra-cerebroventricular infusion in freely-behaving mice during
wakefulness and sleep
Development and maintenance of colonies of genetically-engineered
mice, mouse genotyping (PCR)
Gene expression analysis (qRT-PCR)
Immunoenzymatic/immunofluorescent assays
Advanced mathematic analysis and modelling of biosignals included
spontaneous cardiovascular fluctuations
Software programming (SPSS, Matlab, Labview, Berkeley Madonna) for
customized data acquisition and analysis
Keywords
Cardiorespiratory regulation and sleep
Central autonomic control during sleep
Functional genomics
Selected Publications in the 5 past years
Articles in Peer Review Journals:
Berteotti C., Asti V., Ferrari V., Franzini C, Lenzi P., Zoccoli
G., Silvani A. Central and baroreflex control of heart period
during the wake-sleep cycle in spontaneously hypertensive rats. Am
J Physiol (Regul Integr Comp Physiol.), 293: 293-298, 2007. IF
2010: 3,284
Berteotti C., Franzini C., Lenzi P., Zoccoli G., Silvani A. Surges
of arterial pressure during rapid-eye-movement sleep in
spontaneously hypertensive rats. Sleep, 31: 111-117, 2008. IF 2010:
5,486
Berteotti C., Franzini C, Lenzi P., Magosso E., Ursino M., Zoccoli
G., Silvani A. The baroreflex contribution to spontaneous heart
rhythm assessed with a mathematical model in rats. Autonom.
Neurosci.: Basic and Clinical, 138: 24-30, 2008. IF 2010:
1,671
Silvani A., Grimaldi D., Vandi S., Barletta G., Vetrugno R.,
Provini F., Pierangeli G., Berteotti C., Montagna P., Zoccoli G.,
Cortelli P. Sleep-dependent changes in the coupling between heart
period and blood pressure in human subjects. Am J Physiol (Regul
Integr Comp Physiol.), 294: R1686-92, 2008. IF 2010: 3,284.
Silvani A. Physiological sleep-dependent changes in arterial blood
pressure: central autonomic commands and baroreflex control
(Invited Brief Review). Clin Exp Pharmacol Physiol, 35: 987-94,
2008. IF 2010: 1,960.
Silvani A., Bastianini S., Berteotti C., Franzini C., Lenzi P., Lo
Martire V., Zoccoli G. Sleep modulates hypertension in
leptin-deficient obese mice. Hypertension, 53: 251-255, 2009. IF
2010: 6,908
Silvani A., Bastianini S., Berteotti C., Franzini C., Lenzi P., Lo
Martire V., Zoccoli G. Central and baroreflex control of heart
period during the wake-sleep cycle in consomic rats with different
genetic susceptibility to hypertension. Clin.Exp. Pharmacol.
Physiol., 37:322-327, 2010. IF 2010: 1,960
Silvani A., Bastianini S., Berteotti C., Franzini C., Lenzi P., Lo
Martire V., Zoccoli G. Dysregulation of heart rhythm during sleep
in leptin-deficient obese mice. Sleep, 33:355-361, 2010. IF 2010:
5,486
Plazzi G., Moghadam K.K., Maggi L.S., Donadio V., Vetrugno R.,
Liguori R., Zoccoli G., Poli F., Pizza F., Pagotto U., Ferri R.
Autonomic disturbances in narcolepsy, Sleep Med. Rev, 15: 187-196,
2011. IF 2010: 6.338
Bastianini S., Silvani A., Berteotti C., Elghozi, J.L., Franzini
C., Lenzi P., Lo Martire V., Zoccoli G. Sleep-related changes in
blood pressure in hypocretin-deficient narcoleptic mice. Sleep, 34:
213-218, 2011. IF 2010: 5,486
Silvani A, Magosso E, Bastianini S, Lenzi P, Ursino M. Mathematical
modeling of cardiovascular coupling: central autonomic commands and
baroreflex control Autonom Neurosci: Basic and Clinical,162: 66-71,
2011 IF 2010: 1,671
Bastianini S., Silvani A., Berteotti C., Lo Martire V., Zoccoli
G. High-amplitude theta wave bursts during REM sleep and
cataplexy-like state in hypocretin-deficient narcoleptic mice. J.
Sleep Res. 21: 185-188, 2012. IF 2010: 3,361
Bastianini S., Silvani A., Berteotti C., Lo Martire V., Zoccoli
G. Mice show circadian rhythms of blood pressure during each
wake-sleep state. Chronobiol. Internat. 29: 82–86, 2012. IF
2010: 5,576
Silvani A., Bastianini S., Berteotti C., Lo Martire V., Zoccoli
G Control of cardiovascular variability during undisturbed
wake-sleep behavior in hypocretin-deficient mice . Am J Physiol
Regul Integr Comp Physiol., 302:R958-64, 2012. IF 2010: 3,284.
Lo Martire V., Silvani A., Bastianini S., Berteotti C., Zoccoli
G. Effects of ambient temperature on sleep and cardiovascular
regulation in mice: the role of hypocretin/orexin neurons. PLoS
One, PLoS One 7, e47032, doi: 10.1371/journal.pone.0047032
Chapters in Multi-author Books:
Zoccoli G., Walker A.M. The circulation in sleep in early life;
critical phases in its functional development and neural control.
In: Vegetative Functions and Their Interactions in the Neonate.
Chardon K (Ed), Research Signpost, Trivandrum, Kerala, India, 2010,
pp 141-159.
Zoccoli G, Amici R, Silvani A. The hypothalamus and its functions.
In: Narcolepsy. Pathophysiology, Diagnosis, and Treatment. Baumann
CR, Bassetti CL and Scammell TE (eds). Springer, 1st Edition, 2011,
pp 191-204.
Zoccoli G, Silvani A, Franzini C. Sleep and the peripheral vascular
system. In: Encyclopedia of Sleep. Kushida C et al. (eds),
Elsevier, in press.
Key Publications of the Laboratory
Zoccoli G., Walker A.M., Lenzi P., Franzini C. The Cerebral
Circulation during Sleep: regulation mechanisms and functional
implications. Sleep Med. Rev., 6: 443-455, 2002.
Franzini C. Cardiovascular Physiology: The Peripheral Circulation.
In: Principles and Practice of Sleep Medicine. M.H. Kryger, T.
Roth, W.C. Dement. (Eds), 4th Ed., Elsevier, Philadelphia, 2005, pp
203 - 212.
Silvani A, Lenzi P. Reflex Cardiovascular Control in Sleep. In: The
Physiologic Nature of Sleep. Parmeggiani P.L., Velluti R.A. (Eds),
Imperial College Press, London, 2005, pp. 351-369.
Zoccoli G., Bojic T., Franzini C. Regulation of Cerebral
Circulation during Sleep. In: The Physiologic Nature of Sleep.
Parmeggiani P.L., Velluti R.A. (Eds), Imperial College Press,
London, 2005, pp. 351-369.
Silvani A. Physiological sleep-dependent changes in arterial blood
pressure: central autonomic commands and baroreflex control
(Invited Brief Review). Clin Exp Pharmacol Physiol, 35: 987-94,
2008.
Franzini C. Sleep and Blood Flow. In: Atlas of Clinical Sleep
Medicine, M.H Kryger (Ed), Saunders Elsevier, Philadelphia, 2010,
pp 49-53.
Zoccoli G., Walker A.M. The Circulation in Sleep in Early Life;
critical phases in its functional development and neural control.
In: Vegetative Functions and Their Interactions in the Neonate.
Chardon K (Ed), Research Signpost, Trivandrum, Kerala, India, 2010,
pp 141-159.
Plazzi G., Moghadam K.K., Maggi L.S., Donadio V., Vetrugno R.,
Liguori R., Zoccoli G., Poli F., Pizza F., Pagotto U., Ferri R.
Autonomic disturbances in narcolepsy, Sleep Med. Rev, 15: 187-196,
2011.
Zoccoli G, Amici R, Silvani A. The hypothalamus and its functions.
In: Narcolepsy. Pathophysiology, Diagnosis, and Treatment. Baumann
CR, Bassetti CL and Scammell TE (eds). Springer, 1st Edition, 2011,
pp 191-204.
Zoccoli G, Silvani A, Franzini C. Sleep and the peripheral vascular
system. In: Encyclopedia of sleep. Kushida C et al. (eds),
Elsevier, Philadelphia, in press.