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Giovanna Angela Gentilomi

Associate Professor

Department of Pharmacy and Biotechnology

Academic discipline: MED/07 Microbiology and Clinical Microbiology

Publications

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Publications prior to 2004

M.Musiani, M. Zerbini, G. Gentilomi, M. La Placa. Indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to Epstein-Barr virus capsid antigens and early antigens. Journal of Clinical Microbiology (1986) 23, 302-304.

M. Zerbini, M. Musiani, G. Gentilomi, M. La Placa. "Detection of specific immunoglobulin M antibodies to CMV using monoclonal antibody to immunoglobulin M in an indirect immunofluoresence assay". Journal of Clinical Microbiology. (1986) 24, 166-168.

G. Gentilomi, M. Musiani, M. Plazzi, M. Zerbini. Diagnosi rapida di infezione da cytomegalovirus nei pazienti trapiantati renali, mediante saggio immunocitochimico con fosfatasi alcalina. Patologo Clinico. (1987) 3, 117-119.

M. Musiani, M. Zerbini, G. Frasca`, M. Plazzi, G. Gentilomi, M. La Placa. An immunoalkaline phosphatase staining in the diagnosis of cytomegalovirus infection in renal transplant recipients. In: Transplantation and Clinical Immunology. Volume XVIII. Excerpta Medica (1986) 341.

M. Zerbini, M. Musiani, G. Gentilomi, M. La Placa. Dot immunoperoxidase assay using monoclonal antibody for the direct detection of cytomegalovirus in urine samples. Journal of Clinical Microbiology. (1987) 25, 2197-2199.

M. Musiani, M. Zerbini, S. Ferri, M. Plazzi, G. Gentilomi, M. La Placa. Comparison of immune response to Epstein-Barr virus and cytomegalovirus in sera and synovial fluids of patients with rheumatoid arthritis. Annals of Rheumatic Diseases. (1987) 46, 837-842.

M. Musiani, M. Zerbini, G. Gentilomi, M. La Placa. Heat shock treatment in a rapid assay for cytomegalovirus detection in clinical samples. Serodiagnosis and Immunotherapy (1988) 2, 33-38.

M. Musiani, M. Zerbini, M. Plazzi, G. Gentilomi, and M. La Placa. Immunocytochemical detection of antibodies to Epstein-Barr Virus nuclear antigen by a Streptavidin-Biotin-Complex assay. Journal of Clinical Microbiology. (1988) 26, 1005-1008.

M. Musiani, M. Zerbini, G. Gentilomi and M. La Placa. Rapid quantitative assay of cytomegalovirus infectivity. Journal of Virological Methods. (1988) 20, 333-340

M. Zerbini, G. Gentilomi, M. Musiani. A dot immunoassay with monoclonal antibody to detect viral particles in clinical specimens. In :"Rapid Methods and Automation in Microbiology and Immunology". A. Balows, R.C. Tilton, A. Turano Eds., Brixia Academic Press (1989) 765-769.

M. Musiani, M. Zerbini, G. Gentilomi, M. Plazzi, G. Gallinella, D. Gibellini, M. La Placa. An amplified dot immunoassay for the direct quantitation of adapted and wild strains of human cytomegalovirus.Journal of Virological Methods. (1989) 24, 327-334.

M.Zerbini, M.Musiani, G.Gentilomi, S.Costa, MG Poggi, M.La Placa. Immunological status to Epstein-Barr virus and cytomegalovirus in patients with genital condylomata. European Journal of Epidemiology (1989) 5(3), 343-7.

G. Gentilomi, M. Musiani, M. Zerbini, G. Gallinella, D. Gibellini, and M. La Placa. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes. Journal of Immunological Methods. (1989) 125, 177-183.

M. Musiani, M. Zerbini, A. Muscari, G. M. Puddu, G. Gentilomi, D. Gibellini, G. Gallinella, P. Puddu, M. La Placa. Antibody patterns against cytomegalovirus and Epstein- Barr virus in human atherosclerosis. Microbiologica. (1990) 13, 35-41.

A. Azzi, K. Zakrewska, G. Gentilomi, M. Musiani, M. Zerbini. Detection of B 19 parvovirus infections by a dot -blot hybridization assay using a digoxigenin- labelled probe. Journal of Virological Methods. (1990) 27, 125-134.

M. Musiani, G. Gentilomi, M. Zerbini, D. Gibellini, G. Gallinella, S. Pileri, P. Baglioni, and M. La Placa. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry. (1990) 94, 21-25.

M. Musiani, M. Zerbini, G. Gentilomi, G. Gallinella, S. Venturoli, D. Gibellini, M. La Placa. Rapid detection of cytomegalovirus DNA in urine samples with a dot blot  hybridization immunoenzymatic assay. Journal of Clinical Microbiology. (1990) 28 ,2101-2103.

Zerbini M., M. Musiani, S. Venturoli, G. Gallinella, D. Gibellini, G. Gentilomi, M. La Placa. Rapid screening for B 19 Parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe. Journal of Clinical Microbiology. (1990) 28, 2496-2499.

G.Gentilomi. Il citomegalovirus umano. In : Argomenti di laboratorio per la virologia. Ed. Class International. (1990)

M. Musiani, M. Zerbini, D. Gibellini, G. Gentilomi, M. La Placa, E. Ferri and S. Girotti. Chemiluminescent assay for the detection of viral and plasmid DNAs using digoxigenin labeled probes. Analytical Biochemistry. (1991) 94,394-398.

Musiani, M., Zerbini, M., Gibellini, D., Gentilomi, G., Venturoli, S., Gallinella, G., Ferri, E., and Girotti, S. Chemiluminescent dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera. Journal of Clinical Microbiology. (1991) 29,2047-2050.

Girotti, S., Ferri, E., Ghini, S., Musiani, M., Zerbini, M., Gibellini, D., Gentilomi, G. Direct quantitative assays for the detection of viral DNA. Analytica Chimica Acta .(1991) 255, 387-394, 1991.

Zerbini, M., Musiani, M., Gibellini, D., Gentilomi, G. and La Placa, M. 5-Azacytidine induction of a cellular heat-shock protein and expression of the major immediate early protein of human cytomegalovirus in Vero cells. Microbiologica (1991) 14,287-294.

G. Gentilomi,M. Musiani, M. Zerbini, D. Gibellini, G. Gallinella, S. Venturoli. Double in situ hybridization for detection of herpes simplex virus and cytomegalovirus DNA using non radioactive probes. Journal of Hystochemistry and Cytochemistry. (1992 ) 40, 421-425.

M. Musiani, M. Zerbini, G. Gentilomi, D. Gibellini, G. Gallinella, S. Venturoli, M. La Placa. Detection of CMV DNA in clinical samples of AIDS patients by chemiluminescence hybridization. Journal of Virological Methods. (1992) 38, 1-10.

Zerbini M., Musiani M., Venturoli S., Gallinella G., Gibellini D., Gentilomi G., La Placa M. Different syndromes associated with B19 parvovirus viraemia in paedriatic patients: report of four cases. European Journal of Pediatrics. (1992) 151, 815-817.

Gallinella G., M. Musiani , M. Zerbini, G. Gentilomi, D. Gibellini, S. Venturoli, M. La Placa. Efficient parvovirus B19  DNA purification and molecular cloning. Journal of Virological Methods. (1993) 41, 203-212.

G. Gentilomi, M. Zerbini, M. Musiani, G. Gallinella, D. Gibellini, S. Venturoli, M.C. Re, S. Pileri, C. Finelli, M. La Placa. In situ detection of B19 DNA in bone marrow of immunodeficient patients using a digoxigenin-labelled probe. Molecular and Cellular Probes. (1993) 7, 19-24.

M. Musiani, A. Azzi, M. Zerbini, D. Gibellini, S. Venturoli, K. Zakrzewska, M.C. Re  G. Gentilomi, G. Gallinella , M. La Placa. Nested polymerase chain reaction assay for the detection of B19 Parvovirus DNA in human immunodeficiency virus patients. Journal of Medical Virology. (1993) 40, 157-160.

M. Zerbini, M. Musiani, G. Gentilomi, S. Venturoli, G. Gallinella, D. Gibellini, R. Morandi, B. Guerra, L. Bovicelli, M. La Placa. Symptomatic parvovirus B19 infection of one fetus in a twin pregnancy. Clinical and Infectious Diseases. (1993) 17, 262-263.

G. Cenacchi, M. Musiani, G. Gentilomi, S. Righi, M. Zerbini, J.G. Chandler, C. Scala, M. La Placa, G.N. Martinelli. In situ hybridization at the ultrastructural level: Localization of cytomegalovirus DNA using digoxigenin labelled probes. Journal of Submicroscopic Cytology and Pathology. (1993) 25, 341-345.

M. Zerbini, M. Musiani, D. Gibellini, G. Gentilomi, S. Venturoli, G. Gallinella, M. La Placa. Evaluation of strand-specific RNA probes visualized by colorimetric and chemiluminescent reactions for the detection of B19 parvovirus DNA. Journal of Virological Methods (1993).45, 169-178.

D. Gibellini, M. Zerbini, M. Musiani, S. Venturoli, G. Gentilomi, M. La Placa. Microplate capture hybridization of amplified parvovirus B19 DNA fragment labelled with digoxigenin. Molecular and Cellular Probes. (1993) 7:,453-548.

S. Girotti, E. Ferri, M. Musiani, D. Gibellini, M. Zerbini, A. Roda, G. Gentilomi, S. Venturoli. Detection of viral DNA in hybridization reactions using two chemiluminescent substrates. Clinica Chimica Acta. (1994) 224, 73-80.

G. Nigro, M. Zerbini, A. Krzysztofiak, G. Gentilomi, M.A. Porcaro, T. Mango, M. Musiani. Active or recent parvovirus B19 infection in children with Kawasaki disease. Lancet  (1994) 343,1260-1261.

M. Musiani, M. Zerbini, D. Gibellini, S. Venturoli, G. Gentilomi, G. Gallinella, M. La Placa. Viral diagnosis using hybridization assays with digoxigenin labeled probes. Clinica Chimica Acta (1994) 226, 237-245.

M. Musiani, M. Zerbini, S. Venturoli, G. Gentilomi, V. Borghi, P. Pietrosemoli, M. Pecorari, M. La Placa. Rapid diagnosis of cytomegalovirus encephalitis in patients with AIDS using in situ hybridization. Journal of Clinical Pathology (1994) 47, 886-891.

A. Tosti, M. La Placa, P. Fanti, G. Gentilomi, S. Venturoli, M. Zerbini. M. Musiani. Human papillomavirus type 16 associated periungual squamous cell carcinoma in a patient with acquired immunodeficiency syndrome. Acta Dermato-Venereologica (1994) 74, 478-479.

S. Girotti, M. Musiani, P. Pasini, E. Ferri, G. Gallinella, M. Zerbini, A. Roda, G. Gentilomi, S. Venturoli. Application of a low-light imaging device and chemiluminescent substrates for quantitative detection of viral DNA in hybridization reactions. Clinical Chemistry (1995) 41, 1693-1697.

M. Musiani, M. Zerbini, G. Gentilomi, M. Plazzi,G. Gallinella, S. Venturoli. Parvovirus B 19 clearance from from peripheral blood after acute infection. Journal of Infectious Diseases (1995) 72, 1360-1363.

M. Musiani, M. Zerbini, G. Gentilomi, G. Gallinella, S. Venturoli. PCR assay for the detection of B 19 parvovirus DNA. in: "PCR protocols for diagnosis of human and animal virus diseases." Y. Becker, G. Darai eds. Springer-Verlag Heidelberg, (1995) 289-294.

M.Musiani, M Zerbini, G.Gentilomi, G.Rodorigo, V.De Rosa, D.Gibellini, S.Venturoli, G.Gallinella. Persistent B19 Parvovirus infections in haemophilic HIV-1 patients. Journal of Medical Virology (1995) 46, 103-108.

G.Gallinella, S.Venturoli, G.Gentilomi, M.Musiani ,M.Zerbini. Extent of sequence variability in a genomic region coding for capsid proteins of B19 parvovirus. Archives of Virology (1995) 140, 1119-1125.

M.Zerbini, D.Gibellini, M.Musiani, S.Venturoli, G.Gallinella and G.Gentilomi. Automated detection of digoxigenin-labelled B19 parvovirus amplicons by a capture hybridization assay. Journal of Virological Methods (1995) 55, 1-9.

M. Zerbini, M.Musiani, G. Gentilomi, S. Venturoli, G. Gallinella, R. Morandi. Comparative evaluation of virological and serological methods in prenatal diagnosis of parvovirus  B 19 fetal hydrops. Journal of Clinical Microbiology. (1996) 34, 603-608.

M. Musiani, A. Roda, M. Zerbini, G.Gentilomi, P. Pasini, G.Gallinella, S. Venturoli. Detection of parvovirus B 19 DNA in bone marrow cells by chemiluminescence in situ hybridization. Journal of Clinical Mirobiology. (1996) 34, 1313-1316.

M.Musiani, A.Roda, M.Zerbini, P.Pasini, G.Gentilomi, G.Gallinella, S.Venturoli. Chemiluminecsence in situ hybridization for the detection of cytomegalovirus DNA. American Journal of Pathology. (1996) 148, 1105-1112.

S.Girotti, M.Musiani, E.Ferri, G.Gallinella, M.Zerbini, A.Roda, G.Gentilomi, S.Venturoli. Chemiluminescent immunoperoxidase assay for the dot blot hybridization detection of Parvovirus B19 DNA using a low-light imaging device. Analytical Biochemistry. (1996) 236, 290-295.

M.La Placa Jr, S.Venturoli, G.Gentilomi, M.Zerbini, M.Musiani, A.Tosti. Absence of human papillomavirus DNA in verrucous carcinoma. Dermatology. (1996) 193, 271-272.

A.Tosti, M.La Placa Jr., F.Placucci, G.Gentilomi, S.Venturoli, M.Zerbini, M.Musiani. No correlation between cytomegalovirus and alopecia areata. Journal of Investigative Dermatology. (1996) 107, 443.

G.Gentilomi, M.Musiani , M.Zerbini, G.Gallinella, S.Venturoli, E.Manaresi. Dot immunoperoxidase assay for the detection of Parvovirus B19 antigens in serum samples. Journal of Clinical Microbiology. (1997) 35, 1575-1578.

G.Gentilomi, M.Musiani, A.Roda, P.Pasini, M.Zerbini, G.Gallinella, M.Baraldini, S.Venturoli, E.Manaresi. Co-localization of two different viral genomes in the same sample by double-chemiluminescence in situ hybridization. BioTechniques (1997) 23, 1076-1083.

G.Gallinella, M.Zerbini, M.Musiani, S.Venturoli, G.Gentilomi, E.Manaresi. Quantitation of parvovirus B19 DNA sequences by competitive PCR: differential hybridization of the amplicons and immunoenzymatic detection on microplate. Molecular and Cellular Probes. (1997) 11, 127-133.

M.Musiani, M.Zerbini, S.Venturoli, G.Gentilomi, G.Gallinella, E.Manaresi, M..La Placa Jr, A.D'Antuono, A.Roda, P.Pasini. Sensitive chemiluminescence in situ hybridization for the detection of human papillomavirus genomes in biopsy specimens. Journal of Hystochemistry and Cytochemistry. (1997) 45, 729-735.

G.Gentilomi, M.Musiani, M.Zerbini, G.Gallinella, S.Venturoli, E.Manaresi and R.Morandi. B19 parvovirus induced fetal hydrops: rapid and simple diagnosis by detection of B19 antigens in amniotic fluids. Prenatal Diagnosis. (1998) 18, 363-368.

A.Roda, P.Pasini, M.Baraldini, M.Musiani, G.Gentilomi, C.Robert. Chemiluminescent imaging of enzyme-labeled probes usingan optical microscope-videocamera luminograph. Analitical Biochemistry. (1998) 275(1), 53-62.

S.Venturoli, M.Zerbini, M..La Placa Jr, A.D'Antuono, M.Negosanti, G.Gentilomi, G.Gallinella, E.Manaresi, M.Musiani. Evaluation of immunoassay for the detection and typing of PCR amplified human papillomavirus DNA. Journal of Clinical Pathology. (1998) 58, 143-148.

M.Musiani, P.Pasini, M.Zerbini, A.Roda, G.Gentilomi, G.Gallinella, S.Venturoli, E.Manaresi. Chemiluminescence: a sensitive detection system in in situ hybridization. Hystology and Hystopathology. (1998) 13, 243-248.

M.Zerbini, G.Gentilomi, G.Gallinella, R.Morandi, S.Calvi B.Guerra, M.musiani. Intrauterine parvovirus B19 infection and meconium peritonitis. Prenatal Diagnosis. (1998) 18, 599-606.

S.Venturoli, G.Gallinella, E.Manaresi, G.Gentilomi, M.Musiani, M.Zerbini. IgG response to the immunoreactive region of parvovirus B19 nonstructural protein by immunoblot assay with a recombinant antigen. Journal of Infectious Diseases. (1998) 178, 1826-1829.

I.Orienti, G.Gentilomi, F.Bigucci, B.Luppi, V.Zecchi. Substituted poly(methyl vinyl ether-alt-maleic anhydride) for the release control and targeting of methotrexate. Archiv der Pharmazie. (1998), 331, 347-351.

E. Manaresi, G.Gallinella, M.Zerbini, S.Venturoli, G.Gentilomi, M.Musiani. IgG response to B19 parvovirus VP1 and VP2 linear epitops by immunoblot assay. Journal of Medical Virology. (1999) 57, 174-178.

M.Zerbini, G.Gallinella, E.Manaresi, M.Musiani, G.Gentilomi, S. Venturoli. Standardization of a PCR-ELISA assay in serum samples: diagnosis of active parvovirus B19 infection. Journal of Medical Virology. (1999) 59 (2), 239-44.

E.Manaresi, P.Pasini, G.Gallinella, G.Gentilomi, S.Venturoli, A.Roda, M.Zerbini, M.Musiani. Chemiluminescence western blot assay for the detection of immunity against parvovirus B19 VP1 and VP2 linear epitops using a videocamera based luminograph. Journal of Virological Methods. (1999) 81 (1-2), 91-9.

M.Musiani, P.Pasini, M.Zerbini, G.Gentilomi, A.Roda, G.gallinella, E.Manaresi, S.Venturoli. Prenatal diagnosis of parvovirus B19 induced hydrops fetalis by chemiluminescence in situ hybridization. Journal of Clinical Microbiology. (1999) 37 (7), 2326-9.

L.Garuti. M.Roberti, G.Gentilomi. Synthesis and antiviral assays of some 2-substituted benzimidazole-N-carbamates. Farmaco. (2000) 55 (1), 35-9.

Zerbini M, Cricca M, Gentilomi G, Venturoli S, Gallinella G, Musiani M.Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples.Clinica Chimica Acta. 2000 Dec;302(1-2):79-87

Zerbini M, Gentilomi G, Cricca M, Manaresi E, Bonvicini F, Musiani M. A system to enhance the sensitivity of digoxigenin-labelled probe: detection of B19 DNA in serum samples. Journal of Virological Methods. 2001 Apr;93(1-2):137-44.

Z uffi E, Manaresi E, Gallinella G, Gentilomi GA, Venturoli S, Zerbini M, Musiani M. Identification of an immunodominant peptide in the parvovirus B19 VP1 unique region able to elicit a long-lasting immune response in humans.Viral Immunology. 2001;14(2):151-8.

Venturoli S, Cricca M, Bonvicini F, Gallinella G, Gentilomi G, Zerbini M, Musiani M. Detection of adeno-associated virus DNA in female genital samples by PCR-ELISA. Journal of Medical Virology. 2001 Aug;64(4):577-82

Orienti I, Bigucci F, Gentilomi G, Zecchi V. Self-assembling poly(vinyl alcohol) derivatives, interactions with drugs and control of release. Journal of Pharmaceutical Sciences. 2001 Sep;90(9):1435-44.

Garuti L, Roberti M, Gentilomi G. Synthesis and antiviral assays of some benzimidazole nucleosides and acyclonucleosides. Farmaco. 2001 Nov;56(11):815-9.

Manaresi E, Gallinella G, Gentilomi G, Venturoli S, Zuffi E, Bonvicini F, Cricca M, Zerbini M, Musiani M. Humoral immune response to parvovirus B19 and serological diagnosis of B19 infection. Clinical Laboratory. 2002;48(3-4):201-5.

Gallinella G, Zuffi E, Gentilomi G, Manaresi E, Venturoli S, Bonvicini F, Cricca M, Zerbini M, Musiani M. Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. Journal of Medical Virology. 2003 Sep;71(1):135-9.

Gentilomi G, Zeri L, Ghini S, Zerbini M, Zuffi E, Musiani M, Girotti S. Expression of firefly luciferase gene in Erwinia amylovora. Luminescence. 2003 Jul-Aug;18(4):207-13

 

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