Cesare Rossi

Born in Italy on April 15, 1959.

 

EDUCATION

1983       University of Milano. Student in Biological Sciences. Graduation magna cum laude.

1987       PhD in Molecular and Cellular Biology from the University of Milano

1987       Molecular Biology of Parasitism course (three months) at Woods Hole Laboratory, MA, USA.

2004       Diploma di Specializzazione (PhD equivalent) in Microbiology and Virology from the University of Brescia, Italy.

 

RESEARCH and JOBS

1981-1983    Department of Genetics and Microbiology, University of Milano. Genetics of Meiosis in S. cerevisiae Advisor: Prof. G. Magni.

1984           Department of Genetics and Microbiology, University of Milano. Gene Amplification of the human DHFR gene. Advisor: Prof. A. Ghidoni.

1985-1988    Division of Biology, California Institute of Technology, Pasadena. Genetics and molecular biology of human mitochondria. Advisor: Prof. G. Attardi.

1989           Department of Genetics and Development, Columbia University, New York. Visiting scientist Mouse Genetics, ES cells biology (six months). Advisor: Prof. F. Costantini.

1990-1997    Research contract at the Istituto Zooprofilattico Sperimentale (IZSLER) Brescia, Italy. The IZSLER is the most important institute of the Italian Health Service in the area of animal infectious diseases and food safety. Permanent position as staff scientist from 1992. Research activities at IZSLER included the Molecular biology of caliciviruses (RHDV, EBHSV), Genotyping and diagnosis of Leptospira, diagnosis of pestivirus and lentivirus infection, Development of recombinant vaccines.

1998           Telethon Institute for Gene Therapy, Dibit, HS Raffaele, Milano. (Director Prof. C. Bordignon). Development of retroviral vectors for gene therapy.

1999                 Visiting scientist, Structural Biology Program, European Molecular Biology Laboratory (EMBL), Heidelberg. 3D structure determination and assembly of caliciviruses. Prof. S. Fuller.

2000-2002    Istituto Zooprofilattico Sperimentale (IZSLER), Brescia. Head of the Laboratory for the Study and Diagnosis of Genetically Modified Organisms (GMO lab).

2003-now     -University Hospital S. Orsola-Malpighi, Bologna – Head of the Laboratory of Medical Genetics within the Medical Genetics Unit.(permanent position).

 

TEACHING

1992-1995            Lecturer Scuola di Specializzazione in  Genetica Applicata, Universita’ di Milano e la Scuola di Specializzazione in Biotecnologie, Universita’ di Milano.

1997-1998            Contract as Adjunct Professor, Facolta’ di Scienze, Universita’ di Milano: lectures in molecular virology, course of general microbiology.

1999-2001            Lectures as invited speaker Scuola di Specializzazione in Sanita’ Animale, Facolta’ di Medicina Veterinaria, Universita’ di Parma.

 

2005-2006            University of Bologna Faculty of Sciences: Adjunct Professor of Molecular Microbiology.

2003-2010        University of Bologna Faculty of Medicine: Professor of Medical Genetics (CdL Tecnici di Laboratorio Biomedico)

 

RECENT PUBLICATIONS

G. Lanzi, J.R. de Miranda, M.B. Boniotti, C.E. Cameron, A. Lavazza, L. Capucci, S.M. Camazine and C. Rossi. Molecular and Biological characterization of Deformed wing virus of honey bees  J. Virol. 80, 4998-5009 (2006)

 

Turchetti D., Razzaboni E., Zomer H., Rossi C., Ferrari S., Greco D., Graziano C., Romeo G., Seri M. Psychological consequences of prenatal diagnosis in a case of familial Angelman Syndrome. Prenat Diagn. 26: 1156-1159 (2006)

 

Mantovani V., Garagnani P., Selva P., Rossi C., Ferrari S., Cenci M., Calza N., Cerreta V., Luiselli D., Romeo G.. Simple method for haplotyping the poly(TG) repeat in individuals carrying the IVS8 5T allele in the cystic fibrosis transmembrane conductance regulator gene: a useful tool in genetic counseling. Clinical Chem., 53, 3. (2007)

 

Ferrari S., R. Zuntini, V. Lougaris, A. Soresina, V. Sourkova,M. Fiorini, S. Martino, P. Rossi, M.C. Pietrogrande, B. Martire, G. Spadaro, F. Cardinale, F. Cossu, P. Pierani, I. Quinti, C. Rossi, A. Plebani. Molecular Analysis of the pre-BCR Complex in a Large Cohort of Patients affected by Autosomal Recessive Agammaglobulinemia. Genes and Immunity 8, 325–333 (2007)

 

Pandit B., A Sarkozy, L A Pennacchio, C Carta, K Oishi, S Martinelli, E A Pogna, W Schackwitz, A Ustaszewska, A Landstrom, J M Bos, S R Ommen, G Esposito, F Lepri, C Faul, P Mundel, J P Lo´pez Siguero, R Tenconi, A Selicorni, C Rossi, L Mazzanti, I Torrente, B Marino, M C Digilio, G Zampino, M J Ackerman, B Dallapiccola, M Tartaglia, B D Gelb. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy.  Nat. Genet. 8:1007-12 (2007)

 

Ferrari S, V. Lougaris, S. Caraffi , R. Zuntini, J. Yang, A. Soresina, A. Meini, G. Cazzola, C. Rossi, M. Reth, A. Plebani.  Mutations of the Igß gene cause agammaglobulinemia in man. J. Exp. Med. 204: 2047-20  (2007)

 

Ferrero GB, Baldassarre G, Delmonaco AG, Biamino E, Banaudi E, Carta C, Rossi C, Silengo MC. Clinical and molecular characterization of 40 patients with Noonan syndrome. Eur J Med Genet. 51:566-72. (2008)

 

Sarkozy A, Carta C, Moretti S, Zampino G, Digilio MC, Pantaleoni F, Scioletti AP, Esposito G, Cordeddu V, Lepri F, Petrangeli V, Dentici ML, Mancini GM, Selicorni A, Rossi C, Mazzanti L, Marino B, Ferrero GB, Silengo MC, Memo L, Stanzial F, Faravelli F, Stuppia L, Puxeddu E, Gelb BD, Dallapiccola B, Tartaglia M. Germline BRAF mutations in Noonan, LEOPARD, and cardiofaciocutaneous syndromes: molecular diversity and associated phenotypic spectrum. Hum Mutat. 30: 695-702 (2009)

 

Cordeddu V, Di Schiavi E, Pennacchio LA, Ma'ayan A, Sarkozy A, Fodale V, Cecchetti S, Cardinale A, Martin J, Schackwitz W, Lipzen A, Zampino G, Mazzanti L, Digilio MC, Martinelli S, Flex E, Lepri F, Bartholdi D, Kutsche K, Ferrero GB, Anichini C, Selicorni A, Rossi C, Tenconi R, Zenker M, Merlo D, Dallapiccola B, Iyengar R, Bazzicalupo P, Gelb BD, Tartaglia M.  Mutation of SHOC2 promotes aberrant protein N-myristoylation and causes Noonan-like syndrome with loose anagen hair. Nat Genet. 41:1022-6. (2009)

 

Cirstea IC, Kutsche K, Dvorsky R, Gremer L, Carta C, Horn D, Roberts AE, Lepri F, Merbitz-Zahradnik T, König R, Kratz CP, Pantaleoni F, Dentici ML, Joshi VA, Kucherlapati RS, Mazzanti L, Mundlos S, Patton MA, Silengo MC, Rossi C, Zampino G, Digilio C, Stuppia L, Seemanova E, Pennacchio LA, Gelb BD, Dallapiccola B, Wittinghofer A, Ahmadian MR, Tartaglia M, Zenker M.   A restricted spectrum of NRAS mutations causes Noonan sindrome. Nat Genet. 42:27-9  (2010)

 

Martinelli S, De Luca A, Stellacci E, Rossi C, Checquolo S, Lepri F, Caputo V, Silvano M, Buscherini F, Consoli F, Ferrara G, Digilio MC, Cavaliere ML, van Hagen JM, Zampino G, van der Burgt I, Ferrero GB, Mazzanti L, Screpanti I, Yntema HG, Nillesen WM, Savarirayan R, Zenker M, Dallapiccola B, Gelb BD, Tartaglia M.  Heterozygous germline mutations in the CBL tumor-suppressor gene cause a Noonan syndrome-like phenotype. Am J Hum Genet. 87(2):250-7  (2010)

KNOW-HOW

Managment of diagnostic and research labororatory:

A – Experience as group leader and supervisor of students and technicians.

B – Knowledge of Good Laboratory Practice and set up of diagnostic tests according to the ISO/IEC 17025/45000. From 2003 in charge of quality assurance for the Unit of Medical Genetics (system  9001/Vision2000).

C – Managment of diagnostic actitity for legal, medical and epidemiological purposes. Basic Knoledge of admistrative tools: project planning, problem solving (PDCA), balanced scorecards.

D – Grant writing and budget administration. PI or collaborator in projects from the National Reserch Council Italy, Ministry of Health, European Union, Telethon (private charity).

E – Ability to interact with scientists at the local and international level, to give formal presentations and to write reports, papers and grants in englilsh. Experience in dealing with the administrative director or the general director on all matters regarding the laboratory budget.

Technical expertise:

A – Molecular Biology, Virology, Viral vectors (baculovirus, retroviruses), Cell culture.

B – Expression, purification and study of recombinant proteins.

C – Design and validation of molecular assays for genotyping of bacteria, virus detection and quantitation, GMO, human genetics (various techniques: sequencing, DHPLC, MLPA, qPCR).

D – User of the Mac, Win e Unix OS and specific software for sequence analysis, structure prediction, data mining. User of general purpose software: Msoffice, Filemaker, mySQL, Photoshop.

Languages:

Italian (mother tongue), English (very good), German (basic).