Personal Information
Surname(s)/ First name(s): Ribani Anisa
Place and date of birth: Bologna, 08 October 1987
E-mail: anisa.ribani2@unibo.it
Work Experience
From January 2014: PhD Student in Agricultural, Environmental, Food Science and Technology.
Title of the Project: "Analysis of genome and metabolome in pig and rabbit in order to improve productive performances and feed intake"
Title of Thesis: "Towards the development of nutrigenetic concepts in pigs by merging genomics, transcriptomics and metabolomics"
Main activities and responsibilities: Sequencing of pig genome portions and genes associated with some productive traits related to metabolism and coat colour using Sanger method and NGS (Ion Torrent PGM Platform); sequencing and analysis of trascriptome (RNA-Seq) using Illumina Hi-Seq 2000/2500 and Illumina MiSeq; SNP discovery and analysis in the rabbit and pig genome with NGS and PCR-RFLP method; bioinformatic analysis for read alignments, SNP calling, differential expression patterns. Association studies of candidate genes both in pig and rabbit species.
Supervisor: Prof. Luca Fontanesi
November 2012 - December 2013: Research collaborator with fellowship. Title of the Project: "Study of livestock genomes using next generation sequencing techniques for the identification of molecular markers associated with phenotypic traits." Supervisor: Prof. Luca Fontanesi
Education and training
July – September 2015: Short Term Scientific Mission (STSM) funded by RGB-Net COST Action. Title of the project: “A training period of RNA-Seq data analysis” – TGAC (The Genome Analysis Centre), Norwich (UK). Tutor: Dr. Federica Di Palma (Director of Science, TGAC)
3-6 November 2014: Training School in "Genotyping By Sequencing" - TGAC (The Genome Analysis Centre), Norwich (UK)
16-21 June 2014: Summer School in "Agrobiodiversity of the Mediterranean area: a heritage to rediscover and conserve" - University of Cagliari – Italy. Tutor: Prof. Gianluigi Bacchetta (University of Cagliari)
September 2009 - March 2012: Master Degree in Biodiversity and Evolution – address Animal Biology – Class LM-6 Biology - cum laude - Alma Mater Studiorum University of Bologna. Master Thesis in Molecular Zoology. Thesis title: “The mitochondrial genome of Solemya velum (Mollusca, Bivalvia) and the basal phylogeny of the class Bivalvia.” Supervisor Prof. Marco Passamonti
September 2006 - December 2009: Bachelor Degree in Biology (Class 12) – Address Bioecology -cum laude -Alma Mater Studiorum - University of Bologna. Bachelor Thesis in Animal Physiology. Thesis title: “Hormonal treatment effects of 17-beta-estradiol on larval development and sexual differentiation in Bufo bufo (L.)” Supervisor: Prof Francesco Zaccanti
Research Experiences and Technical skills
Wet-lab: DNA and RNA extraction and purification from different biological tissues; DNA amplification with PCR and bacterical trasformation; quality control of DNA and RNA samples using BioAnalyzer and qPCR; genotyping by PCR-RFLP; Sanger sequencing; next generation sequencing (Ion Torrent PGM and Illumina HiSeq and MiSeq); preparation of libraries and running of a NSG experiment (Ion Torrent and Illumina platforms).
Bioinfomatic skills: Biological databases and bioinformatic tools (NCBI, BLAST, ENSEMBL, SwissProt, UniProt, MitoZoa, Plink). DNA sequence analysis and read alignment softwares (Galaxy, CodonCode, MEGA, SeaView, CLC Sequence Viewer, IGV, ClustalW, MAFFT, bwa). SNP calling software for NGS (SAMtools). RNA-seq data analysis softwares (TopHat, Clufflinks). Phylogeny and population genetics softwares (MrBayes, Past, Arlequin, Structure). Basic knowledge of R.
Scientific interest
I am a biologist mainly interested in evolutionary and molecular biology of several animals, both wild and domesticated. My attention is focused mainly in the pig species studying the domestication process, including how artificial selection shaped their genomes, their physiology and their productive traits.
Congresses
- 30-31 January 2017: 3th TREASURE Meeting, Bologna, Italy;
- 12 October 2016: 4th National Meeting of Mora Romagnola breed, Brisighella (RA), Italy;
- 29 August – 2 September 2016: 67th EAAP Annual Meeting (European Federation of Animal Science), Belfast, UK
- 7-8 April 2016: CASTRUM Meeting (Tender SANCO/2014/G3/026), Bologna, Italy;
- 3-5 November 2015: Final RGB-Net meeting + 6th Rabbit Biotechnology Meeting, Lyon, France
- 21-22 September 2015: Lagomorph genomic consortium (LaGomiCs) meeting, Bologna, Italy
- 9-12 June 2015: XXI ASPA Congress (Animal Science and Production Association), Milan (Italy)
- 6-8 May 2014: 3rd RGB-Net meeting and satellite meetings, Zagreb (Croatia)
- 11-13 June 2013: XX ASPA Congress (Animal Science and Production Association). Bologna (Italy)
- 18-19 February 2013: 1st RGB-Net-WLS Lagomorph genomics workshop, Bologna (Italy)
- 5-8 September 2011: LXXII National Congress of Italian Zoological Union. Bologna (Italy)
- 24-27 April 2010: 2nd International Symposium on Genomics of Plant Genetic Resources, Bologna (Italy)
Oral presentations in Scientific Congresses
- Next generation semiconductor based sequencing of bitter taste receptor genes in different pig breeds and populations and association study of identified polymorphisms using a DNA pooling strategy - XXI ASPA Congress (Animal Science and Production Association) – Milan (Italy) 9-12 June 2015
- Analysis of variability of the TYR gene in wild and domestic rabbits - RGB-Net Seminars and Meetings – Zagreb (Croatia) 7-8 May 2014
Poster in Scientific Congresses
- Utzeri V.J., Ribani A., Schiavo G., Bertolini F., Geraci C., Bovo S., Fontanesi L. Food metagenomics against frauds: applications of next generation semiconductor based sequencing on meat and dairy products and honey – FoodInnova 2017 – 31 January – 3 February 2017 – Cesena (Italy)
- Ribani A., Utzeri V.J., Geraci C., Dall’Olio S., Nanni Costa L., Fontanesi L. Local pig breeds are “less domesticated” than commercial populations: evidences from variability in the MC1R and NR6A1 genes in Italian autochthonous breeds – 9th International Symposium on Mediterranean Pig – Portalegre (Portugal) – 3-5 November 2016
- Ribani A., Bertolini F., Schiavo G., Scotti E., Utzeri V.J., Dall’Olio S., Trevisi P., Bosi P., Fontanesi L. Next generation semiconductor based sequencing of bitter taste receptor genes in different pig populations and association study using a selective DNA pool-seq approach – 67th EAAP Annual Meeting (European Federation of Animal Science) – Belfast (UK) – 29 August – 2 September 2016
- Ribani A., Bertolini F., Schiavo G., Scotti E., Utzeri V.J., Dall’Olio S., Trevisi P., Bosi P., Fontanesi L. A next generation semiconductor based target re-sequencing DNA pool-seq approach for the identification of SNPs and association studies: application to bitter taste receptor genes in different pig populations - 35th ISAG (International Society for Animal Genetics) Conference – Salt Lake City (USA) 23-27 July 2016
- Schiavo G., Hoffmann O.I., Ribani A., Utzeri V.J., Ghionda M.C., Bovo S., Fontanesi L. A genomic landscape of mitochondrial DNA insertions in the nuclear pig genome - 35th ISAG (International Society for Animal Genetics) Conference – Salt Lake City (USA) 23-27 July 2016
- Fontanesi L., Bovo S., Schiavo G., Mazzoni G., Ribani A., Utzeri V.J., Dall’Olio S., Bertolini F., Fanelli F., Mazzullo M., Galimberti G., Calò D.G., Trevisi P., Pagotto U., Bosi P. Deconstructing the pig genome-metabolome functional interactions – 35th ISAG (International Society for Animal Genetics) Conference – Salt Lake City (USA) 23-27 July 2016
- Ribani A., Utzeri V.J., Schiavo G., Bovo S., Geraci C., Fontanesi L. Food genomics: application of innovative DNA analysis technologies for authentication of food products. - TRADEIT Entrepreneurship Summer Academy – Postdam (Germany) 6-10 June 2016
- Utzeri V.J., Ribani A., Dall’Olio S., Scotti E., Veličković N., Fontanesi L. Evidence of introgression of domesticated genes in several European wild boar populations XXI ASPA Congress (Animal Science and Production Association) – Milan (Italy) 9-12 June 2015
- Utzeri V.J., Bertolini F., Ribani A., Dall’Olio S., Fontanesi L. Identification of the causative mutation of the albinism determining the white coat colour of the Asinara donkey breed - XXI ASPA Congress (Animal Science and Production Association) – Milan (Italy) 9-12 June 2015
- Trevisi P., Ribani A., Colombo M., Utzeri V.J., Bosi P., Fontanesi L. A first nutrigenomic trial in pigs identifies a DNA polymorphism affecting kynurenine metabolites after tryptophan addition and E. coli challenge, 13th Digestive Physiology of Pigs – Kliczkow (Poland) 19-21 May 2015
- Plazzi F., Ribani A., Passamonti M. The complete mitochondrial genome of Solemya velum (Mollusca: Bivalvia) and its relationships with Conchifera, V SIBE Congress (Italian Society for Evolutionary Biology) – Trento (Italy) 28-31 August 2013
- Ribani A., Utzeri V.J., Scotti E., Bertolini F., Dall’Olio S., Fontanesi L. Sequence analysis of the tyrosinase gene (TYR, Albino locus) in wild and domesticated rabbits (Oryctolagus cuniculus) and in other wild Lagomorph species, XX ASPA Congress (Animal Science and Production Association) – Bologna (Italy) 11-13 June 2013.
- Bertolini F., Schiavo G., Scotti E., Ribani A., Martelli P.L., Casadio R., Fontanesi L. Application of the Ion Torrent technology to identify single nucleotide polymorphisms in the rabbit genome XX ASPA Congress (Animal Science and Production Association) – Bologna (Italy) 11-13 June 2013
Pubblications
- Fontanesi L., Bertolini F., Scotti E., Schiavo G., Colombo M., Trevisi P., Ribani A., Buttazzoni L., Russo V, Dall'Olio S. (2014) Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs. Animal Biotechnology 2014 26 (2): 92-97
- Bertolini F., Schiavo G., Scotti E., Ribani A., Martelli P.L., Casadio R., Fontanesi L. (2014) High throughput SNP discovery in the rabbit (Oryctolagus cuniculus) genome by next generation semiconductor based-sequencing. Animal Genetics 2014, 45 (2):304-307
- Fontanesi L., Ribani A., Scotti E., Utzeri V.J., Velickovic N., Dall’Olio S. (2014) Differentiation of meat from European wild boars and domesticated pigs using polymorphisms in the MC1R and NR6A1 genes. Meat Science 2014, 98: 781-784.
- Utzeri V.J., Ribani A., Fontanesi L. (2014) A premature stop codon in exon 2 of the TYRP1 gene is associated with brown coat colour in rabbits (Oryctolagus cuniculus). Animal Genetics 2014, 45:600 - 603
-Plazzi F., Ribani A., Passamonti M. (2013) The complete mitochondrial genome of Solemya velum (Mollusca: Bivalvia) and its relationships with Conchifera, BMC Genomics 2013, 14:409
