Education and Training
November 2014– October 2017
PhD in Biomedical Science and Biotechnology (XXX cycle)
Activity: Cell cultures, Cell transfections, Real Time PCR, FRET, Expression of recombinant proteins, Luciferase test, Proliferation tests, ChIP, Chromatography, Electrophoresis, Microscopy, Western Blot, RNA extraction and purification, Circular Dichroism, Spectroscopy, RNA Footprinting, Biotin-avidin Pull-down assay, Luciferase assay, Experience in using radioisotopes (32P) (Tutor: Prof. Luigi E. Xodo)
October 2007-July 2013
Master Degree in Pharmaceutical Chemistry and Technology (105/110)
Activity: Experimental thesis entitled 'Bioavailability of different Coenzyme Q10 formulations in cultured cells'. Experience in cell cultures (T67 human neuroblastome, H9c2 rat cardiomyocites, HeLa,Fibroblasts, HDF, TPC1, Hek 293);Fractionating of subcellular organelles (mitochondria);Oxygen consumption evaluation with polarimetric assays;Viability assays (MTT assay, Trypan blue assay);Liquid-liquid extraction of compounds of interest (Coenzyme Q10 e.g.);HPLC analysis and spectrophotometric detection of interesting compounds (CoQ, ATP, ADP, AMPetc); Enzimatic kinetics (cytrate synthase e.g);Protein evaluation assays (Lowry protein assay, Biuret test). (Tutor: Prof. Romana Fato)
Working Experience
- January 2020- December 2022
University of Bologna, Department of Pharmacy and Biotechnology FaBiT
FIRC-AIRC three-years fellowship "Antonio Di Gennaro" (Rif. 23953)
(Tutor: Prof. Giovanni Capranico)
- January 2018- December 2019
University of Bologna, Department of Pharmacy and Biotechnology FaBiT
Research contract (Assegno di ricerca)
(Tutor: Prof. Giovanni Capranico)
University of Udine, Department of Medicine
Work contract (Collaborazione coordinata e continuativa Co.co.co)
Activity:Cell cultures, Real Time PCR, FRET, Expression of recombinant proteins, Luciferase test, Proliferation tests, ChIP, Chromatography, Electrophoresis, Microscopy, Western Blot
AWARDS
PhD Journal Club Competition (Università of Udine, Department of Medical Area)
Poster selected for TRAVEL GRANT at SIBBM annual Conference- Nucleic acid immunity: from cellular mechanism to new technologies- 11-13 June
TEACHING EXEPERIENCE
University of Udine: Laboratory Assistance to foreign visiting students of the Ecole de Biologie Industrielle, University of Cergy Pontoise, France
University of Udine: Alternanza Scuola-Lavoro-Campus Biomedico Macro Area Biologica e della Fisiologia
University of Bologna: Bando contratti di tutorato FarmaBio a.a. 2018/19 - Bologna e Imola, selezione per l'attribuzione di una attività di tutorato relativa all'insegnamento denominato BIOLOGIA MOLECOLARE [cod. 67004] - [Modulo 4] Modulo 4 - laboratorio eucariotica dell'Anno Accademico 2018/2019 per il corso BIOTECNOLOGIE
University of Bologna: Bando contratti di tutorato FarmaBio a.a. 2018/19 - Bologna e Imola, Selezione per l'attribuzione di una attività di tutorato relativa all'insegnamento denominato BASI MOLECOLARI E GENETICHE DELLE MALATTIE CON LABORATORIO [cod. 67161] - [Modulo 2] Laboratorio di Struttura della cromatina dell'Anno Accademico 2018/2019 per il corso BIOTECNOLOGIE FARMACEUTICHE
University of Bologna: Bando contratti di tutorato FarmaBio a.a. 2019/20 - Bologna e Imola, selezione per l'attribuzione di una attività di tutorato relativa all'insegnamento denominato BIOLOGIA MOLECOLARE [cod. 12946] - [Modulo 3] Modulo3 - laboratorio eucariotica dell'Anno Accademico 2019/2020 per il corso BIOTECNOLOGIE
University of Bologna: Bando contratti di tutorato FarmaBio a.a. 2019/20 - Bologna e Imola, selezione per l'attribuzione di una attività di tutorato relativa all'insegnamento denominato GENOMICS OF DISEASES (I.C.) [cod. 90816] - MOLECULAR MECHANISMS OF DISEASES [cod. 90817] per il corso PHARMACEUTICAL BIOTECHNOLOGY
University of Bologna: Co-supervisor of master thesis
Peer-reviewed publications
Miglietta G., Gouda AS. , Cogoi S. , Pedersen EB. and Xodo LE. (2015) Nucleic Acid Targeted Therapy: G4 Oligonucleotides Downregulate HRAS in Bladder Cancer Cells through a Decoy Mechanism. ACS Medicinal Chemistry Letters 6 (12): 1179–1183
Miglietta G. , Cogoi S. , Pedersen EB. and Xodo LE. (2015) GC-elements controlling HRAS transcription form i-motif structures unfolded by heterogeneous ribonucleoprotein particle A1. Scientific Reports 5: 18097
Miglietta G., Cogoi S., Marinello J. , Capranico G. , Tikhomirov AS. , Shchekotikhin A. and Xodo LE. RNA G-quadruplexes in Kirsten ras (KRAS) oncogene as targets for small molecules inhibiting translation. Journal of Medicinal Chemistry doi: 10.1021/acs.jmedchem.7b00622
Cogoi S., Ferino A. , Miglietta G. , Pedersen EB. And Xodo LE. The regulatory G4 motif of the Kirsten ras (KRAS) gene is sensitive to guanine oxidation: implications on transcription. Nucleic Acid Research. 2018 Jan 25;46(2):661-676. doi: 10.1093/nar/gkx1142
Ferino A., Miglietta G., Picco R, Vogel S., Wengel J.,XodoLE. MicroRNA therapeutics: design of single-stranded miR-216b mimics to target KRAS in pancreatic cancer cells. [https://www.ncbi.nlm.nih.gov/pubmed/30306823] RNA Biology 2018;15(10):1273-1285. doi: 10.1080/15476286.2018.1526536
Amato J., Miglietta G., Morigi R., Iaccarino N., Locatelli A., Leoni A., Novellino E., Pagano B., Capranico G. and Randazzo A., 2020. Mono-hydrazone based G-quadruplex selective ligands induce DNA damage and genome instability in human cancer cells.Journal of Medicinal Chemistry 2020; 63(6): 3090-3103 doi: 10.1021/acs.jmedchem.9b01866
Conference Abstracts
G. Miglietta, S. Cogoi, L.E. Xodo The 5’-UTR of human KRAS mRNA contains a G4 cluster recognized by small molecules blocking translation, EMBO | EMBL SYMPOSIUM: THE COMPLEX LIFE , Heidelberg (DE) 5-10 October 2016
G. Miglietta, S. Cogoi, L.E. Xodo, Targeted gene therapy for pancreatic cancer: anthraquinone derivatives bind to G-quadruplex cluster in KRAS mRNA and suppress translation, 6TH INTERNATIONAL MEETING ON QUADRUPLEX NUCLEIC ACIDS, Prague (CZ) 31 May and 1st June 2017
G. Miglietta, S. Cogoi, L.E. Xodo, Molecular strategies to suppress oncogenic KRAS in pancreatic cancer cells: use of G4 RNA binders and unlocked nucleic acid modified miRNAs, PHD STUDENTS MEETING: LIFE SCIENCES FOR A BETTER FUTURE, Santa Margherita Ligure (IT), 11-13 May 2017
A. TikhomirovI, G. Miglietta, S. Cogoi, A. ShchekotikhinI, L.E. Xodo, G quadruplex RNA in Kirsten ras transcript as promising target for anticancer therapy ,THE 43 RD FEBS CONGRESS, Prague (CZ) 7-12 July 2018
A. De Magis, S.G. Manzo, G. Miglietta, J. Marinello, M. Russo, O. Sordet, R. Morigi and G. Capranico, R-loops and micronuclei mediate cellular effects of G-quadruplex ligands in human
Cancer cells, NUCLEIC ACIDS, IMMUNITY AND GENOME DEFENCE, Brno (CZ) 4-6 September 2018
R. Morigi, J. Amato, N. Iaccarino, B. Pagano, A. Locatelli, A. Leoni, M. Rambaldi, G. Miglietta, J. Marinello, G. Capranico, E. Novellino, A. Randazzo, Targeting human telomeric and promoter G-quadruplexes: synthesis, biophysical and biological studies of new g uanylhydrazones as potential antitumor agents, BIONIC 2018: BIOLOGY OF NON-CANONICAL NUCLEIC ACIDS:from humans to pathogens ,Padua (IT) 26-28 September 2018
G. Miglietta, Jessica Marinello, R. Morigi, A. Locatelli, A. Leoni, G. Capranico, Development of new G4 binders in cancer therapy: synthesis, biophysical and biological studies of hydrazones derivatives, SIBBM2019 –Frontiers in molecular biology- Nucleic acid immunity: from cellular mechanism to new technologies- Bologna (IT) 11-13 June 2019
