The invention relates to a method that enables rapid and sensitive molecular detection of the genome by detecting nucleic acids thereof.
| Patent title | Method of detecting a target genome |
|---|---|
| Thematic area | Health |
| Ownership | ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA, Università di Messina |
| Inventors | Sabrina Conoci, Salvatore Petralia, Enrico Rampazzo, Alessandra Zanut, Francesco Paolucci, Luca Prodi, Giovanni Valenti |
| Filed on | 13 July 2021 |
Current analytical methodologies for the detection of pathogenic microorganisms are in vitro diagnostic (IVD) methods based on:
a. Detection of antibodies induced by these microorganisms in the host's immune system (immunological tests).
b. Molecular detection of genomic sequences using PCR-based technologies.
These amplification methods can lead to false positives and make nucleic acid quantification challenging. The elimination of the amplification process is an essential condition for the development of advanced molecular diagnostic systems for nucleic acids in Point-of-Care (PoC) settings. The so-called Point-of-Care Technologies (POCT) represent the future of medical diagnostics.
New electrochemiluminescent analytical method for the PCR-free quantification of RNA genomes of pathogenic microorganisms."
Advantages:
- Rapid and direct determination (< 1h)
- High analytical performance
- Versatile methodology applicable to various pathogens, which can be detected during the same analytical process
- Capability to detect ds-DNA of pathogens (DNA viruses such as HBV)