Academic Year 2018/2019
- Docente: Patrizia Brigidi
- Credits: 6
- SSD: CHIM/11
- Language: Italian
- Moduli: Patrizia Brigidi (Modulo 1) Patrizia Brigidi (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Pharmaceutical Biotechnology (cod. 8519)
Learning outcomes
Aim of the course is the employment of microorganisms for the production of metabolites as well as recombinant proteins of pharmaceutical interest.
Course contents
Genetic improvement of microbial strains. Industrial production of
microbial primary and secondary metabolites: amino acids, vitamins,
enzymes, antibiotics and antitumoral agents. Advanced screening to
discover new antibiotics. Expression and production of heterologous
pharmaceutical proteins in microbial hosts: hormones, fibrinolytic
agents, anticoagulants, growth factors, cytokines, recombinant
toxins, vaccines, biologicals. Protein engineering to improve
pharmacokynetics of recombinant proteins.
The laboratory course will address the following experiences:
fermentative production of tetracycline by Streptomyces
aureofaciens and assessment of the antibiotic yield; assessment of
bacterial endotoxins by LAL test; molecular phylogenetics.
Readings/Bibliography
Scientific papers and reviews suggested by the teacher Laboratory notes describing the experimental procedures.
Teaching methods
Development and employment of genetically modified microorganisms in pharmaceutical field.
Assessment methods
Students will be tested with an oral exam. The latter will verify the knowledge about microorganisms used for the industrial production of drugs
Teaching tools
PC, projector, laboratory
Office hours
See the website of Patrizia Brigidi