- Docente: Silvia Turroni
- Credits: 6
- SSD: CHIM/11
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Applied Pharmaceutical Sciences (cod. 8518)
Learning outcomes
Aim of the course is the knowledge of microbial ecosystems, of the main traditional and molecular techniques for microbial analysis, including -omics technologies, and of the main tests for control. The experimental methods will be acquired through lab exercises.
Course contents
Analysis of microbial communities by culture-dependent methods.
Analysis of microbial communities by culture-independent molecular methods.
Biotechnology, fermentation and microbial metabolism.
Selection, screening and genetic improvement of industrial microorganisms.
Industrial fermentation processes: batch, fed-batch and repeated fed-batch processes.
Industrial fermentation processes: continuous and continuous processes with recycling.
Microbial production of primary metabolites: amino acids and vitamins.
Microbial production of secondary metabolites: antibiotics.
Production of metabolites partially linked to growth: organic acids.
Production of microbial enzymes.
Microbial bioconversions using enzymes and immobilized cells.
Industrial applications.
Microbes and microbiomes for bioremediation.
Sterilization: principles and methods.
Laboratory: microscopy, isolation and identification of microorganisms from environmental samples, extraction of genomic DNA from a bacterial culture, amplification, sequence analysis by comparison with databases.
Readings/Bibliography
Slides and notes taken during the lessons are essential for exam preparation. As a reference, the student can deepen the topics on the following texts:
Biotecnologie Microbiche. Stefano Donadio, Gennaro Marino. Casa Editrice Ambrosiana.
Microbiologia Sistematica, Ambientale ed Industriale. Prescott (2). McGraw-Hill.
Microbiologia Industriale. Matilde Manzoni. Casa Editrice Ambrosiana.
LABORATORY NOTES with the operational description of the experiences.
Teaching methods
The course consists of 6 CFU of which 4 CFU of lectures with the support of explanatory slides, and 2 CFU of laboratory exercises.
Assessment methods
Oral exam in correspondence with the appeals fixed in the examination windows.
Teaching tools
PC, projector, laboratory experiences.
Office hours
See the website of Silvia Turroni
SDGs
This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.