37188 - Histology and Embryology Laboratory (C2D)

Learning outcomes

At the end of the course, the student is familiar with the laboratory with various methodologies, including histochemistry and immunohistochemistry, for the study of cellular and subcellular structures at the light microscopy . In particular, the student is able to recognize the morphological aspects that allow the diagnosis of various human tissues in their peculiarities.

Course contents

Techniques for the study of histology: light and electron microscopy. Techniques for the preparation of materials for histological observation. Importance of cell shape and size. Strategies for the identification under light microscope of covering and glandular exocrine and endocrine epithelial tissues; Connective tissue classification (mucous mature, fibrillar period, reticular, elastic, dense, unilocular and multilocular adipose); cartilage (hyaline, fibrous and elastic); bone (lamellar and non-lamellar spongy and compact; tooth: cement and dentin; direct and indirect ossification), blood and lymphoid tissue; muscle (smooth, striated skeletal and cardiac), and nerve tissue (in the central and peripheral nervous system).

Readings/Bibliography

- Guida illustrata all'istologia di C. Rizzoli, M.A. Brunelli, C. Castaldini; Piccin Editore

- Istologia atlante di G. Bani, D. Bani, T. Bani Sacchi; Idelson-Gnocchi Editore

- Atlante di Istologia di Gianpaolo Papaccio; Idelson-Gnocchi Editore

- Dongmei Cui Atlante di Istologia con correlazioni funzionali e cliniche a cura di A. Filippini, A. Musarò, E. Ziparo; Piccin Editore

- Atlante di Istologia e Anatomia microscopica di M.H. Ross, W. Pawlina, T. A. Barnash; Casa Editrice Ambrosiana.

-Anatomia microscopica Atlante Manrico Morroni Casa Editrice Edi-Ermes

-Anatomia microscopica del Netter a cura di A.Baldi CIC Edizioni Internazionali

Teaching methods

Projections of histological slides with an indication of identification strategies. Individual exercises to the optical microscope, to groups of 30 students, guided by the teacher with the aim of providing to each student the opportunity to engage in self-recognition of the histological preparations.

Assessment methods

The learning assessment will include the examination of slides at light microscopy that is introductory to the oral exam. The students that have not passed are not admitted to the oral exam. The oral exam is about Cytology, Histology and Embryology in according the contents published in Histology and Embryology 1 and Histology and Embryology 2 courses. A low mark in one of the three parts precludes the whole exam. The student can choose to keep the mark of examination of slides (valid until December 2015) or to repeat it. In the opinion of the examiners, the final assessment will be the weighed average of the three results.
The online registration to the examination is mandatory.

Teaching tools

Mono and binocular optical microscopes, optical microscope with 5 heads to delve into the details histology with small groups of students, optical microscope connected to the projector via a computer, power point presentations. Histological slide.
There are histological virtual display with tools and simulation programs of the microscope. A lesson will give you details for access.

Office hours

See the website of Furio Pezzetti