- Docente: Barbara Roda
- Credits: 6
- SSD: CHIM/01
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Chemistry and Materials Chemistry (cod. 8006)
Learning outcomes
Use and application of quantitative instrumental analysis to chemical and bioanalytical problem: spettrcoscopic techniques (UV-vis, MS), separative techniques (HPLC and field-flow fractionation), chemiluminescence, enzymatic tests, immunoassays. Different analytical assay format, data elaboration and report.
Course contents
Spectroscopy: Uv-vis, atomic absorption, chemiluminescence.
Separative techniques: HPLC (reverse-phase HPLC, size exclusion chromatography, ionic chromatography); field-flow fractionation
Analysis of complex samples: digestion; matrix effect determination.
Methods for the determination of protein concentration.
Enzymatic analysis: Kinetics of enzyme-catalyzed reactions - Measurement of an enzyme catalitic activity - Measurement of the concentration of an enzymatic substrate.
Immonological methods: Competitive and non competitive immunoassays - Homogeneous and heterogeneous immunoassays - Immobilization of antibodies - Synthesis of enzymatic, fluorescent, or chemiluminescent tracers and detection methods - Development and optimization of an immunoassay.
General aspects for gene analysis.
Readings/Bibliography
L. Spandrio "Principi e Tecniche di Chimica Clinica", Piccin
-A.J. Ninfa, D.P. Ballou "Metodologie di Base per la Biochimica e la Biotecnologia", Zanichelli (consultazione)
-J.M. Berg, J.L. Tymoczko, L. Stryer "Biochimica", V Edizione, Zanichelli
Analytical Chemistry by Open Learning: Quantitative Bioassay, D. Hawcroft, T. Hector, F. Rowell, John Wiley and Sons i Dosaggi immunoenzimatici, E. Ishikawa, T. Kawai, K. Miyai, Piccin
Teaching methods
Room lessons assisted by projected material available to students on electronic media from the web.
Training in laboratory
Data elaboration and report preparation
Assessment methods
Laboratory report with data anlysis. Final oral exam with discussion on reports and on arguments presented during the lessons.
Teaching tools
Power Point slides and Word report available on the net http://campus.unibo.it/
Office hours
See the website of Barbara Roda