Academic Year 2017/2018

  • Teaching Mode: Traditional lectures
  • Campus: Bologna
  • Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 8859)

Course contents

REGENERATIVE MEDICINE AND GENOMICS (C.I.) A.A. 2017/2018

GENOMICS

Auletta Multimediale, Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale,

Sede Operativa di Istologia, Embriologia e Biologia Applicata, Via Belmeloro 8, 40126 Bologna (BO).

Attending 70% of the lessons/laboratories is required.

Lessons (24 hours)

rational background of common methods in Genomics

1. Human Genomics and Post-Genomics [WED 11 OCTOBER 11, 2017, 14:30-17:00]

2. Bibliography and Genomics databases [WED OCTOBER 18, 2017, 14:30-17:00]

3. Methods for studying nucleic acids (Extraction, Electrophoresis, Enzymes) [THU OCTOBER 19, 2017, 14:30-17:00]

4. Nuclic Acids Hybridization (Southern Blot, Northern Blot, Microarray) [THU OCTOBER 26, 2017, 14:30-17:00]

5. Polymerase Chain Reaction (Kinetics, Parameters, Design) [WED NOVEMBER 08, 2017, 14:30-17:00]

6. cDNA, EST and genomic Libraries [WED NOVEMBER 15, 2017, 14:30-17:00]

7. Nucleic Acids Sequencing and Sequence Analysis [WED 22 NOVEMBER 23, 2017, 15:00-17:30]

8. Computational and System Biology - Trisomy 21 [TUE NOVEMBER 28, 2017, 14:30-17:00]

Laboratory (15 hours)

1. Advanced use of bibliography and genomics databanks [WED OCTOBER 25, 2017, 14:30-17:00]

2. Gene structure - NCBI Gene database [THU NOVEMBER 02, 2017, 14:30-17:00]

3. PCR primer design [THU NOVEMBER 09, 2017, 14:30-17:00]

4. Sequence analysis [THU NOVEMBER 23, 2017, 14:30-17:00]

5. Documentary and discussion [WED JANUARY 10, 2018, 14:30-17:00]

Readings/Bibliography

Fantoni A. et al. Genetica. Piccin, 2009 (Chapter 33: "Human Genome Analysis" - in Italian).

Sito: http://apollo11.isto.unibo.it/

Assessment methods

Short written essay, to be sent as a file via e-mail within the day of the examination.

Teaching tools

Availability of 19 personal computers (iMac) in the "Auletta Multimediale".

Office hours

See the website of Pierluigi Strippoli