03766 - Immunology (LZ-B)

Course contents

 

General properties of the immune system. Nomenclature, cells of the immune system. Primary and secondary lymphoid organs. Anatomy and functions of lymphoid tissues.

Leukocyte recruitment in the tissues and their recirculation in the lymphoid organs. Adhesion molecules, chemokines and leukocyte migration.

Innate immunity. Epithelial barriers. The phagocytes: neutrophils and macrophages. Phagocytosis, bactericidal activity. Dendritic cells. Natural Killer cells. The complement system. Receptors of innate immunity: PRR (Pattern Recognition Receptors). Innate immune system cytokines (interleukins and interferons). Role of innate immunity in the stimulation of the adaptive immune responses.

Adaptive immunity. Antigen and its features. Structure, development and function of the lymphocyte receptors for the antigen: immunoglobulins and T cell receptor (TCR). Mechanisms of recombination of the genes of immunoglobulins and of the TCR. Intracellular signal transduction pathways. Selection and clonal expansion of B and T lymphocytes

Recognition, processing and presentation of antigens. Structure and function of the major histocompatibility complex (MHC). Cells presenting protein antigens (APC): dendritic cells, macrophages and B cells. Physiological significance of antigen presentation in association with MHC molecules.

T and B lymphocyte response to antigens: activation and differentiation. Clonal expansion. Role of accessory and costimulatory molecules in the immune response. Effector and memory lymphocytes. Cytokines. T-dependent and T-independent antigens. T-B cooperation in the antibody response. Germinal center reaction. Affinity maturation and class exchange of antibodies. The different classes of helper T cells: Th1, Th2, Th17, follicular Th and regulatory T cells. Effector mechanisms of humoral immunity (antibodies, complement) and cellular immunity (NK cells, cytotoxic and helper T lymphocytes).

Mucose-associated immunity. Development and functions of immunity associated to the intestinal mucosa. Role of the microbiota. Immunity associated with the epidermis and pulmonary immunity.

Immune tolerance. Central and peripheral tolerance in T and B cells. Role of clonal selection and costimulation. Role of infections in breaking tolerance.

An integrated view of the responses to pathogens. Strategies of response to bacteria, viruses, fungi and to helminths. Escape strategies of microorganisms.

Vaccines. Vaccines made with attenuated or killed virus or bacteria. Vaccines made with microbial components, or with inactivated toxins. Adjuvants.

AB0 and Rh systems. Structure and genetics of AB0 antigens. "Natural" antibodies and compatibility of transfusions. Rh incompatibility and fetal erythroblastosis.


Transplant rejection. Molecular and cellular basis of the recognition of alloantigens: role of the MHC. Direct and indirect rejection of transplants. Hyperacute, acute and chronic rejection. Bone marrow transplantation and graft versus host disease (GVHD).

Immunodeficiencies. Congenital immunodeficiencies of the innate and adaptive immune system. Severe combined immune deficiencies (SCID). Immunodeficiencies of the T and B compartments. Short notes on the pathogenesis of AIDS.

Hypersensitivity reactions. Immediate hypersensitivity (type I): the role of IgE. Anaphylactic reactions. Hypersensitivity type II: reaction of antibodies against surface antigens. Hypersensitivity Type III: immune complexes, serum sickness. Delayed hypersensitivity type IV.

Immunological techniques. Monoclonal antibodies. Enzyme-Linked Immunosorbent Assay (ELISA). Western blot. Fluorescence-Activated Cell Sorting (FACS).

 

 

 

A.K. Abbas e A:H: Lichtman, , Immunologia Cellulare e Molecolare, Ed. Elsevier-Masson VIII edition.

 

Teaching methods

Slides presentation

Office hours

See the website of Mariella Chiricolo