39291 - Clinical Pathology Techniques

Academic Year 2017/2018

  • Teaching Mode: Traditional lectures
  • Campus: Bologna
  • Corso: First cycle degree programme (L) in Biomedical Laboratory techniques (cod. 8484)

Learning outcomes

At the end of the course the student will acquire the principles used for the critical evaluation and interpretation of some clinical laboratory methods.

Course contents

A brief history of the cardiovascular system; short survey on the microanatomy of the cardiovascular system: arteries, veins and capillaries. Research methodologies for the study of stem cells resident in the vascular wall. Vascular-wall stem cells. Isolation protocols. Mechanical and enzymatic methods. Cell culture. Characteristics of cell growth in culture. Culture media. Instrumentation. Main vascular cell immunephenotypes.  Clusters of Differentiation. Methods for detecting surface antigens. Cell reprogramming. Nuclear transfer. IPSC. Subletal stress. Genetic editing. 3D cultures. Cell Immortalization.

Readings/Bibliography

  • G Pasquinelli - S Valente. Sistema vascolare e cellule staminali del sistema vascolare. In: Cellule Staminali. GP Bagnara (ed.) II ed. Ed. Esculapio, Bologna, 2016. pp 51-77.
  • G. Pasquinelli. Lo studio morfologico ed immunocitochimico della parete vascolare. In: Metodologie di Ricerca in Chirurgia Vascolare. Gianluca Faggioli (ed.). Edizioni Minerva Medica. Torino. 1996. pp 7-22.
  • Microscopie in Biologia e Medicina. C. Scala, G. Pasquinelli, G. Cenacchi. CLUEB - Bologna 1995.

Teaching methods

The course consists of two CFU: lectures, supplementary activities by experts of the technical area of teaching.

Assessment methods

The final exam consists of the oral presentation of two questions on the program's topics.
For each question, the student receives a vote based on the demonstrated ability to briefly expose the issues with some critical thinking. The weighted result, expressed in 30/30, contributes in proportion to the number of credits, the final vote of the entire integrated course.

Teaching tools

The teaching material presented in class will be made available on amsacta.unibo.it in electronic format.

Office hours

See the website of Gianandrea Pasquinelli